Bub3 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76796

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Bub3 Antibodies »

Product Summary for Bub3 Antibody - BSA Free

Immunogen

Antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human bub3. The immunogen is located within the last 50 amino acids of Bub3. Amino Acid Squence: SYMYEMDDTEHPEDG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Bub3 Antibody - BSA Free

Western Blot: Bub3 AntibodyBSA Free [NBP1-76796]

Western Blot: Bub3 AntibodyBSA Free [NBP1-76796]

Western Blot: Bub3 Antibody [NBP1-76796] - Jurkat cell lysate with bub3 antibody at (A) 0.5 and (B) 1 ug/ml.
Immunocytochemistry/ Immunofluorescence: Bub3 Antibody - BSA Free [NBP1-76796]

Immunocytochemistry/ Immunofluorescence: Bub3 Antibody - BSA Free [NBP1-76796]

Immunocytochemistry/Immunofluorescence: Bub3 Antibody [NBP1-76796] - Immunofluorescence of bub3 in Jurkat cells with bub3 antibody at 20 ug/mL.
Immunocytochemistry/ Immunofluorescence: Bub3 Antibody - BSA Free [NBP1-76796]

Immunocytochemistry/ Immunofluorescence: Bub3 Antibody - BSA Free [NBP1-76796]

Immunocytochemistry/Immunofluorescence: Bub3 Antibody [NBP1-76796] - Jurkat cells with Bub3 antibody at 10 ug/ml.

Applications for Bub3 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10-20 ug/ml

Western Blot

0.5-1 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Bub3

The mitotic checkpoint protein Bub3 is involved with the essential spindle checkpoint pathway which operates during early embryogenesis. Bub3 is important during G2 and early mitosis stages, permitting entry into mitosis depending upon the assembly state of microtubules, thus preventing premature sister chromatid separation, mis-segregation and aneuploidy. Bub3 contains four WD repeat domains and is required for the kinetochore localization of Bub1, a related kinase that is necessary for spindle assembly checkpoint function. Bub1 is able to autophosphorylate and can catalyze the phosphorylation of Bub3. Both Bub1 and Bub3 are mutually dependent for function. Altered Bub expression levels may significantly impair mitotic checkpoint function and is associated with tumor cell proliferation.

Long Name

BUB3 Budding Uninhibited By Benzimidazoles 1 Homolog

Alternate Names

BUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog, BUB3 budding uninhibited by benzimidazoles 3 homolog, BUB3L, budding uninhibited by benomyl, budding uninhibited by benzimidazoles 3 homolog (yeast), hBUB3, mitotic checkpoint component, mitotic checkpoint protein BUB3

Gene Symbol

BUB3

UniProt

O43684

Additional Bub3 Products

Product Documents for Bub3 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Bub3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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