BubR1 Antibody - C-terminus

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-30805

Novus Biologicals, part of Bio-Techne
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NBP3-30805-250ug
NBP3-30805-50ug
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Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all BubR1 Antibodies »

Product Specifications

Immunogen

Recombinant protein corresponding to amino acids 883-1052 of the mouse BubR1 protein.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for BubR1 Antibody - C-terminus

BubR1 Antibody - C-terminus

Western Blot: BubR1 Antibody - C-terminus [NBP3-30805] -

Western Blot: BubR1 Antibody - C-terminus [NBP3-30805] - Immunoprecipitation/Western blot analysis using Bub1b-CT antibody on NIH/3T3 cells synchronized to obtain mostly mitotic cells as determined by flowcytometry.

Applications for BubR1 Antibody - C-terminus

Application
Recommended Usage

Immunoprecipitation

: 1-10 ug/ml

Western Blot

: 1-10 ug/ml
Application Notes
Antibody detects Bub1b in mitotic cells seperated from NIH/3T3 cells by IP/Westen blot. Detects a 130 kDa band..
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

Phosphate buffered saline

Preservative

0.08% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BubR1

Mitotic checkpoint serine/threonine-protein kinase BUB1 beta (BUB1B), also known as BubR1, is a kinase involved in spindle checkpoint function. BUBR1 is essential for normal mitotic progression as it prevents cells from entering into anaphase too early. BUBR1 has been detected in human cancers. BubR-1 localizes to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation.

BubR1 antibodies are useful tools for cancer research and cell division studies.

Alternate Names

Bub1A, BUBR1budding uninhibited by benzimidazoles 1 (yeast homolog), beta, budding uninhibited by benzimidazoles 1 homolog beta (yeast), EC 2.7.11.1, hBUBR1, MAD3/BUB1-related protein kinase, MAD3LBUB1beta, Mitotic checkpoint kinase MAD3L, Protein SSK1, SSK1mitotic checkpoint serine/threonine-protein kinase BUB1 beta

Gene Symbol

BUB1B

Additional BubR1 Products

Product Documents for BubR1 Antibody - C-terminus

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BubR1 Antibody - C-terminus

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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