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c-Maf Antibody (BLR045F)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14672

Recombinant Monoclonal
Novus Biologicals, part of Bio-Techne
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NBP3-14672

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen (Negative), Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # BLR045F

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for c-Maf Antibody (BLR045F)

Immunogen

between 125 and 175

Clonality

Monoclonal

Host

Rabbit

Scientific Data Images for c-Maf Antibody (BLR045F)

Western Blot: c-Maf Antibody (BLR045F) [NBP3-14672]

Western Blot: c-Maf Antibody (BLR045F) [NBP3-14672]

Western Blot: c-Maf Antibody [NBP3-14672] - Antibody: Rabbit anti-c-Maf recombinantmonoclonal antibody used at1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure timeof 30 seconds. Lower Panel: Rabbit anti-Myc Tagrecombinant monoclonal antibody.
Immunohistochemistry: c-Maf Antibody (BLR045F) [NBP3-14672]

Immunohistochemistry: c-Maf Antibody (BLR045F) [NBP3-14672]

Immunohistochemistry: c-Maf Antibody [NBP3-14672] - Sample: FFPE section of mouse renal cell carcinoma.Antibody: Rabbit anti-c-MAF recombinant monoclonalantibody used at 1:125.Secondary: HRP-conjugated goat anti-rabbit IgG Substrate: DAB.
Western Blot: c-Maf Antibody (BLR045F) [NBP3-14672]

Western Blot: c-Maf Antibody (BLR045F) [NBP3-14672]

Western Blot: c-Maf Antibody [NBP3-14672] - Whole cell lysate (50 ug) from RPMI-8226, Hep-G2, 786-O, and Jurkat cells prepared using NETN lysis buffer. Rabbit anti-c-Maf recombinant monoclonal antibody (NBP3-14672lot 3) used at 1:1000. HRP-conjugated goat anti-rabbit IgG (NB7156). Chemiluminescence with an exposure time of 30 seconds.

Applications for c-Maf Antibody (BLR045F)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 to 1:500

Immunohistochemistry

1:100 to 1:500

Immunohistochemistry-Frozen (Negative)

1:100 to 1:500

Immunoprecipitation

6 µl/1 mg lysate

Western Blot

1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

>95%

Formulation

Borate Buffered Saline (BBS) pH 8.2 with , BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: c-Maf

c-Maf is part of the Maf family of transcription factors that possess a conserved basic region and a leucine zipper domain that mediate DNA and protein-protein binding and plays an important role in tissue-specific gene regulation and cell differentiation. c-Maf is a transcriptional activator of interleukin 4 and 10 in T-cells, and is involved in lens fiber cell differentiation. c-Maf translocation has been observed in human multiple myeloma and may be oncogenic.

Long Name

v-maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog (Avian)

Alternate Names

CCA4, cMaf, MAF2

Gene Symbol

MAF

Additional c-Maf Products

Product Documents for c-Maf Antibody (BLR045F)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for c-Maf Antibody (BLR045F)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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