c-Myb Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-61662

Novus Biologicals, part of Bio-Techne
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NB100-61662
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all c-Myb Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QRHYNDEDPEKEKR corresponding to internal region according to NP_001123645.1, NP_005366.2, NP_001123644.1, NP_001155128.1, NP_001155129.1, NP_001155130.1, NP_001155131.1.

Epitope

QRHYNDEDPEKEKR

Specificity

This antibody is expected to recognize isoforms 1-7 only (Numbering from NCBI RefSeq). (NP_001123645.1; NP_005366.2; NP_001123644.1; NP_001155128.1; NP_001155129.1; NP_001155130.1; NP_001155131.1)

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for c-Myb Antibody

Western Blot: c-Myb Antibody [NB100-61662]

Western Blot: c-Myb Antibody [NB100-61662]

Western Blot: c-Myb Antibody [NB100-61662] - Staining of MOLT4 cell lysate (35 ug protein in RIPA buffer). Antibody at 2 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: c-Myb Antibody [NB100-61662]

Immunohistochemistry-Paraffin: c-Myb Antibody [NB100-61662]

Immunohistochemistry-Paraffin: c-Myb Antibody [NB100-61662] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
Immunohistochemistry-Paraffin: c-Myb Antibody [NB100-61662]

Immunohistochemistry-Paraffin: c-Myb Antibody [NB100-61662]

Immunohistochemistry-Paraffin: c-Myb Antibody [NB100-61662] - Staining of paraffin embedded Human Kidney. Antibody at 4 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Applications for c-Myb Antibody

Application
Recommended Usage

Immunohistochemistry

4 ug/mL

Immunohistochemistry-Paraffin

4 ug/mL

Peptide ELISA

Detection limit 1:4000

Western Blot

1 - 3 ug/mL
Application Notes
Western blot: Approx 70 kDa band observed in lysates of cell line MOLT4 and in preliminary testing of Human Bone Marrow and Lymph Node lysate (calculated MW of 72.3kDa according to NP_005366.2).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: c-Myb

Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. Myb is a transcription factor related to Myc, and is expressed primarily in haematopoietic cells and immature gastroepithelial cells.

Alternate Names

Cmyb, c-myb, c-myb protein (140 AA), c-myb_CDS, c-myb10A_CDS, c-myb13A_CDS, c-myb14A_CDS, c-myb8B_CDS, efg, Proto-oncogene c-Myb, transcriptional activator Myb, v-myb avian myeloblastosis viral oncogene homolog, v-myb myeloblastosis viral oncogene homolog (avian)

Entrez Gene IDs

4602 (Human)

Gene Symbol

MYB

UniProt

NP_005366.2 (Human)

Additional c-Myb Products

Product Documents for c-Myb Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for c-Myb Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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