c-Rel Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB600-474
Key Product Details
Species Reactivity
Validated:
Human, Mouse (Negative)
Applications
ELISA, Gel Super Shift Assays, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit Serum
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for c-Rel Antibody
Immunogen
c-Rel peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH). (Uniprot: Q04864)
Reactivity Notes
This antibody may react non-specifically with other proteins. Control peptide will compete only with the specific reaction of antiserum with the c-Rel subunit.
Localization
Nuclear
Specificity
Anti-c-Rel may react non-specifically with other proteins. Control peptide will compete only with the specific reaction of antiserum with the c-Rel subunit.
Clonality
Polyclonal
Host
Rabbit
Isotype
Serum
Description
This product was prepared from monospecific antiserum by delipidation and defibrination
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for c-Rel Antibody
![Western Blot: c-Rel Antibody [NB600-474]](https://resources.bio-techne.com/images/products/c-Rel-Antibody-Western-Blot-NB600-474-img0005.jpg "Western Blot: c-Rel Antibody [NB600-474]")
Western Blot: c-Rel Antibody [NB600-474]
Western Blot: c-Rel Antibody [NB600-474] - All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1 h. Washes with TBS preceded color development.
c-Rel Antibody
Western blot of HeLa cell extract. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1 h. Washes with TBS preceded color development.Applications for c-Rel Antibody
Application
Recommended Usage
ELISA
1:5000-1:25000
Gel Super Shift Assays
1:500
Western Blot
1:500-1:3000
Application Notes
This product was tested by ELISA and by immunoblot. This product is suitable for the detection by immunoblot of human NFkB cRel at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) . No reaction was observed against the analogous Mouse protein. This product was also tested in a gel supershift assay and found to be reactive against all cRel containing human NFkB complexes using 0.5 to 1.0 ul per assay.
Simple Western Antibody Database for Simple Western validation: tested in HEK-/+UV, whole cell lysate; separated by charge, antibody dilution of 1:50.
Simple Western Antibody Database for Simple Western validation: tested in HEK-/+UV, whole cell lysate; separated by charge, antibody dilution of 1:50.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Delipidation and Defibrination
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: c-Rel
Long Name
Cellular Repressor of E1A-stimulated Genes
Alternate Names
cRel, REL
Gene Symbol
REL
UniProt
Additional c-Rel Products
Product Documents for c-Rel Antibody
Product Specific Notices for c-Rel Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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