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C12orf45 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-93474

Novus Biologicals, part of Bio-Techne
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NBP1-93474
NBP1-93474-25ul

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LINSQPKSRKTSTLQTVRIERSPLLDQVQTFLPQMARANEKLRKEMAAAPPGRFNIENIDGPHSKVIQMDVALFEMNQSDSKEVD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for C12orf45 Antibody

Western Blot: C12orf45 Antibody [NBP1-93474]

Western Blot: C12orf45 Antibody [NBP1-93474]

Western Blot: C12orf45 Antibody [NBP1-93474] - Analysis in control (vector only transfected HEK293T lysate) and C12orf45 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: C12orf45 Antibody [NBP1-93474]

Immunocytochemistry/ Immunofluorescence: C12orf45 Antibody [NBP1-93474]

Immunocytochemistry/Immunofluorescence: C12orf45 Antibody [NBP1-93474] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear membrane.
Immunohistochemistry-Paraffin: C12orf45 Antibody [NBP1-93474]

Immunohistochemistry-Paraffin: C12orf45 Antibody [NBP1-93474]

Immunohistochemistry-Paraffin: C12orf45 Antibody [NBP1-93474] - Staining of human stomach shows moderate cytoplasmic and nuclear positivity in glandular cells.

Applications for C12orf45 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: C12orf45

Alternate Names

chromosome 12 open reading frame 45, MGC40397

Gene Symbol

NOPCHAP1

Additional C12orf45 Products

Product Documents for C12orf45 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for C12orf45 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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