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C16orf72 Antibody (OTI2D1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02850

Novus Biologicals, part of Bio-Techne
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NBP2-02850
Conjugate
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2D1

Concentration

0.65 mg/ml

Product Summary for C16orf72 Antibody (OTI2D1)

Immunogen

Full length human recombinant protein of human C16orf72 (NP_054836) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

30.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for C16orf72 Antibody (OTI2D1)

Western Blot: C16orf72 Antibody (OTI2D1) [NBP2-02850]

Western Blot: C16orf72 Antibody (OTI2D1) [NBP2-02850]

Western Blot: C16orf72 Antibody (2D1) [NBP2-02850] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C16orf72 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C16orf72.
Immunocytochemistry/Immunofluorescence: C16orf72 Antibody (2D1) [NBP2-02850] Staining of COS7 cells transiently transfected by pCMV6-ENTRY C16orf72.
Flow Cytometry: C16orf72 Antibody (OTI2D1) [NBP2-02850]

Flow Cytometry: C16orf72 Antibody (OTI2D1) [NBP2-02850]

Flow Cytometry: C16orf72 Antibody (2D1) [NBP2-02850] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-C16orf72 antibody, and then analyzed by flow cytometry.

Applications for C16orf72 Antibody (OTI2D1)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.65 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: C16orf72

Alternate Names

chromosome 16 open reading frame 72, FLJ41272, hypothetical protein LOC29035, PRO0149

Entrez Gene IDs

29035 (Human)

Gene Symbol

C16orf72

Additional C16orf72 Products

Product Documents for C16orf72 Antibody (OTI2D1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for C16orf72 Antibody (OTI2D1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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