C9orf72 Antibody (GT1553-RB)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13658
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # GT1553-RB
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human C9orf72. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Centrifuge briefly prior to opening.
Scientific Data Images for C9orf72 Antibody (GT1553-RB)
![Western Blot: C9orf72 Antibody (GT1553-RB) [NBP3-13658]](https://resources.bio-techne.com/images/products/C9orf72-Antibody-GT1553-RB-Western-Blot-NBP3-13658-img0001.jpg "Western Blot: C9orf72 Antibody (GT1553-RB) [NBP3-13658]")
Western Blot: C9orf72 Antibody (GT1553-RB) [NBP3-13658]
Western Blot: C9orf72 Antibody (GT1553-RB) [NBP3-13658] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT1553-RB] (NBP3-13658) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: C9orf72 Antibody (GT1553-RB) [NBP3-13658] -
Western Blot: C9orf72 Antibody (GT1553-RB) [NBP3-13658] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [1553-RB] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301 was used to detect the primary antibody.
ICC/IF-C9orf72 Antibody (GT1553-RB) [NBP3-13658] -
C9orf72 Antibody (1553-RB) [NBP3-13658] - DIV10 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: C9orf72 stained by C9orf72 antibody [1553-RB] diluted at 1:250.Red: Tau, a cytoskeleton marker, stained by Tau antibody [287] diluted at 1:500.Blue: Fluoroshield with DAPI.
Applications for C9orf72 Antibody (GT1553-RB)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Assay dependent
Immunohistochemistry
Assay dependent
Immunohistochemistry-Paraffin
Assay dependent
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: C9orf72
Long Name
Chromosome 9 Open Reading Frame 72
Alternate Names
DENNL72, FTDALS1
Gene Symbol
C9orf72
Additional C9orf72 Products
Product Documents for C9orf72 Antibody (GT1553-RB)
Product Specific Notices for C9orf72 Antibody (GT1553-RB)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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