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CA125/MUC16 Antibody (CL2782)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59023

Novus Biologicals, part of Bio-Techne
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NBP2-59023
NBP2-59023-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL2782

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for CA125/MUC16 Antibody (CL2782)

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence:GVLVTTRRRKKEGEYNVQQQCPGYYQSHLDLEDLQ

Epitope

NVQQQCPGYYQSHLD

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CA125/MUC16 Antibody (CL2782)

CA125/MUC16 Antibody (CL2782)

Immunohistochemistry-Paraffin: CA125/MUC16 Antibody (CL2782) [NBP2-59023] -

Immunohistochemistry-Paraffin: CA125/MUC16 Antibody (CL2782) [NBP2-59023] - Analysis in human cervix, uterine and liver tissues using NBP2-59023 antibody. Corresponding MUC16 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: CA125/MUC16 Antibody (CL2782) [NBP2-59023]

Immunocytochemistry/ Immunofluorescence: CA125/MUC16 Antibody (CL2782) [NBP2-59023]

Immunocytochemistry/Immunofluorescence: CA125/MUC16 Antibody (CL2782) [NBP2-59023] - Staining of HeLa cells using the Anti-MUC16 monoclonal antibody, showing specific staining in the plasma membrane and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
CA125/MUC16 Antibody (CL2782)

Immunohistochemistry-Paraffin: CA125/MUC16 Antibody (CL2782) [NBP2-59023] -

Immunohistochemistry-Paraffin: CA125/MUC16 Antibody (CL2782) [NBP2-59023] -Staining of human cervix, uterine shows moderate membranous positivity in glandular cells.

Applications for CA125/MUC16 Antibody (CL2782)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CA125/MUC16

MUC16 (CA125) is a serum marker that is used routinely in gynecologic practice to monitor patients with ovarian cancer. It is a mullerian duct differentiation antigen that is over expressed in epithelial ovarian cancer cells and secreted into the blood, although its expression is not entirely confined to ovarian cancer. Most biochemical studies have concluded that MUC16 is a high molecular mass glycoprotein, although estimates of its size range from 200 to 2000 kDa with smaller "subunits" being described by some investigators.

Long Name

Ovarian Cancer Antigen CA125

Alternate Names

MUC16

Gene Symbol

MUC16

UniProt

Additional CA125/MUC16 Products

Product Documents for CA125/MUC16 Antibody (CL2782)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CA125/MUC16 Antibody (CL2782)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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