We are setting up an ELISA type of assay for CA125. On your web page: Applications: Uses: Detection of CA125 antigen. Suggested pairs for sandwich immunoassay (capture-detection): NB120-10032 (capture) with NBP2-11624 or NB600-1468 as detection. Can you send me more details how these antibodies have been working in your sandwich immunoassay?

Name: CA125/MUC16 Antibody (X75)
Brand: Novus Biologicals
SKU: NB600-1468

These antibodies have been tested with very general sandwich ELISA, without any assay optimization, just to determine which MAbs work together as pairs. Coat plate with NB120-10032 in alkaline carbonate buffer Post-coat with casein Add HRP-conjugated NBP2-11624 and antigen to plate Incubate microplates for 45 min at room temperature with shaking Wash 5 times with saline-Tween Add 100 ul of substrate Stop enzymatic reaction addition of 5% sulphuric acid. Measure the optical density. The sensitivity without optimization has been around 5 IU/ml. But we really do not have any recommended protocol and the suitability and sensitivity of the Abs should be determined in your own assay.

CA125/MUC16 Antibody (X75)

Novus Biologicals, part of Bio-Techne | Catalog # NB600-1468

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Product Datasheet / COA / SDS

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Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Sandwich ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # X75

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CA125/MUC16 Antibodies »

Product Specifications

Immunogen

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with CA125 antigen purified from human ovarian carcinoma (MW > 1 MDa).

Epitope

B1 epitope group

Localization

Cell Membrane

Specificity

CA125 antigen, epitope specificity group B (ISOBM classification) similar to M-11.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Applications for CA125/MUC16 Antibody (X75)

Application

Recommended Usage

Sandwich ELISA

1:100-1:2000

Western Blot

1-5 ug/ml

Application Notes

Detection of CA125 antigen. Suggested pairs for sandwich immunoassay (capture-detection): NB120-10032 - NB600-1468.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Ion exchange chromatography

Formulation

10mM Tris (pH 7.5) and 0.15M NaCl

Preservative

0.05% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CA125/MUC16

MUC16 (CA125) is a serum marker that is used routinely in gynecologic practice to monitor patients with ovarian cancer. It is a mullerian duct differentiation antigen that is over expressed in epithelial ovarian cancer cells and secreted into the blood, although its expression is not entirely confined to ovarian cancer. Most biochemical studies have concluded that MUC16 is a high molecular mass glycoprotein, although estimates of its size range from 200 to 2000 kDa with smaller 'subunits' being described by some investigators.

Long Name

Ovarian Cancer Antigen CA125

Alternate Names

MUC16

Entrez Gene IDs

94025 (Human)

Gene Symbol

MUC16

UniProt

Q8WXI7

Additional CA125/MUC16 Products

Product Documents for CA125/MUC16 Antibody (X75)

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for CA125/MUC16 Antibody (X75)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CA125/MUC16 Antibody (X75)

Novus Biologicals, part of Bio-Techne | Catalog # NB600-1468

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

Concentration

View all CA125/MUC16 Antibodies »

Product Specifications

Immunogen

Epitope

Localization

Specificity

Clonality

Host

Isotype

Applications for CA125/MUC16 Antibody (X75)

Sandwich ELISA

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: CA125/MUC16

Long Name

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional CA125/MUC16 Products

Product Documents for CA125/MUC16 Antibody (X75)

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for CA125/MUC16 Antibody (X75)