CA19-9/Sialyl Lewis A Antibody (GT252)

Name: CA19-9/Sialyl Lewis A Antibody (GT252)
Brand: Novus Biologicals
SKU: NBP3-13651

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13651

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # GT252

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CA19-9 Antibodies »

Product Specifications

Immunogen

The immunogen used to generate this antibody corresponds to CA19-9/Sialyl Lewis A.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Description

Centrifuge briefly prior to opening.

Scientific Data Images for CA19-9/Sialyl Lewis A Antibody (GT252)

Western Blot: CA19-9/Sialyl Lewis A Antibody (GT252) [NBP3-13651]

Western Blot: CA19-9/Sialyl Lewis A Antibody (GT252) [NBP3-13651] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CA19-9/Sialyl Lewis A antibody [GT252] (NBP3-13651) diluted at 1:1000 and competitor's antibody diluted at 1:250. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.

Immunocytochemistry/ Immunofluorescence: CA19-9/Sialyl Lewis A Antibody (GT252) [NBP3-13651]

Immunocytochemistry/Immunofluorescence: CA19-9/Sialyl Lewis A Antibody (GT252) [NBP3-13651] - CA19-9/Sialyl Lewis A antibody [GT252] detects CA19-9/Sialyl Lewis A protein at cell membrane by immunofluorescent analysis. Sample: SW1116 (high expression) and HT29 (low expression) cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CA19-9/Sialyl Lewis A stained by CA19-9/Sialyl Lewis A antibody [GT252] (NBP3-13651) diluted at 1:1000 and competitor's antibody diluted at 1:1000. Blue: Fluoroshield with DAPI.

Flow Cytometry: CA19-9/Sialyl Lewis A Antibody (GT252) [NBP3-13651]

Flow Cytometry: CA19-9/Sialyl Lewis A Antibody (GT252) [NBP3-13651] - CA19-9/Sialyl Lewis A antibody [GT252] (NBP3-13651) detects CA19-9/Sialyl Lewis A by flow cytometry analysis. Sample: COLO 205 cell. Black: Unlabelled sample was used as a control. Red: CA19-9/Sialyl Lewis A antibody [GT252] (NBP3-13651) dilution: 1:50. Blue: Highly cited antibody [116-NS-19-9] dilution: 1:50. Acquisition of 20,000 events were collected for FACS analysis.

Western Blot: CA19-9/Sialyl Lewis A Antibody (GT252) [NBP3-13651]

Western Blot: CA19-9/Sialyl Lewis A Antibody (GT252) [NBP3-13651] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CA19-9/Sialyl Lewis A antibody [GT252] (NBP3-13651) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.

ELISA: CA19-9/Sialyl Lewis A Antibody (GT252) [NBP3-13651]

ELISA: CA19-9/Sialyl Lewis A Antibody (GT252) [NBP3-13651] - Competition analysis of CA 19-9 antibody [GT252] (NBP3-13651) (330 ng/mL) binding to immobilized recombinant HSA-CA 19-9 (coated at 3 ug/mL) in the presence of soluble Lewis A or Lewis X tetrasaccharides (1.0-0.01 mM). Bound antibody was detected by Goat Anti-Mouse IgG antibody (HRP) (NBP2-19382) (1:10000).

Sandwich ELISA: CA19-9/Sialyl Lewis A Antibody (GT252) [NBP3-13651] - Sandwich ELISA detection of Colo 205 and Jurkat whole cell extracts using CA19-9/Sialyl Lewis A antibody [GT252] (NBP3-13651) as capture antibody at concentration of 5 ug/mL and HRP-conjugated Ca19-9 antibody [GT8510] (NBP3-13652) as detection antibody at concentration of 1 ug/mL. Please notice that NBP3-13652 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-13651. Please contact us for custom HRP-conjugated antibody.

Immunohistochemistry-Paraffin: CA19-9/Sialyl Lewis A Antibody (GT252) [NBP3-13651] -

Immunohistochemistry-Paraffin: CA19-9/Sialyl Lewis A Antibody (GT252) [NBP3-13651] - CA19-9/Sialyl Lewis A antibody [GT252] detects CA19-9/Sialyl Lewis A protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human pancreatic cancer.CA19-9/Sialyl Lewis A stained by CA19-9/Sialyl Lewis A antibody [GT252] (NBP3-13651) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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