CA19-9/Sialyl Lewis A Antibody (GT933) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13650
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Non-species specific
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Microarray, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # GT933
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
The immunogen used to generate this antibody corresponds to CA19-9/Sialyl Lewis A.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
Centrifuge briefly prior to opening.
Scientific Data Images for CA19-9/Sialyl Lewis A Antibody (GT933) - Azide and BSA Free
Western Blot: CA19-9/Sialyl Lewis A Antibody (GT933) [NBP3-13650]
Western Blot: CA19-9/Sialyl Lewis A Antibody (GT933) [NBP3-13650] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CA19-9/Sialyl Lewis A antibody [GTX933] (NBP3-13650) diluted at 1:1000 and competitor's antibody diluted at 1:250. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.Immunocytochemistry/ Immunofluorescence: CA19-9/Sialyl Lewis A Antibody (GT933) [NBP3-13650]
Immunocytochemistry/Immunofluorescence: CA19-9/Sialyl Lewis A Antibody (GT933) [NBP3-13650] - CA19-9/Sialyl Lewis A antibody [GT933] detects CA19-9/Sialyl Lewis A protein at cell membrane by immunofluorescent analysis. Sample: SW1116 (high expression) and HT29 (low expression) cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CA19-9/Sialyl Lewis A stained by CA19-9/Sialyl Lewis A antibody [GT933] (NBP3-13650) diluted at 1:1000 and competitor's antibody diluted at 1:1000. Blue: Fluoroshield with DAPI.Flow Cytometry: CA19-9/Sialyl Lewis A Antibody (GT933) [NBP3-13650]
Flow Cytometry: CA19-9/Sialyl Lewis A Antibody (GT933) [NBP3-13650] - CA19-9/Sialyl Lewis A antibody [GT933] (NBP3-13650) detects CA19-9/Sialyl Lewis A by flow cytometry analysis. Sample: COLO 205 cell. Black: Unlabelled sample was used as a control. Red: CA19-9/Sialyl Lewis A antibody [GT933] (NBP3-13650) dilution: 1:50. Blue: Highly cited antibody [116-NS-19-9] dilution: 1:50. Acquisition of 20,000 events were collected for FACS analysis.Applications for CA19-9/Sialyl Lewis A Antibody (GT933) - Azide and BSA Free
Application
Recommended Usage
ELISA
Assay dependent
Flow Cytometry
Assay dependent
Immunocytochemistry/ Immunofluorescence
Assay dependent
Immunohistochemistry
Assay dependent
Immunohistochemistry-Paraffin
Assay dependent
Microarray
Assay dependent
Sandwich ELISA
Assay dependent
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CA19-9
Long Name
Carbohydrate Antigen 19-9
Alternate Names
CA 19-9
Additional CA19-9 Products
Product Documents for CA19-9/Sialyl Lewis A Antibody (GT933) - Azide and BSA Free
Product Specific Notices for CA19-9/Sialyl Lewis A Antibody (GT933) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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