CAB39 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15658

Novus Biologicals, part of Bio-Techne
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NBP2-15658
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Xenopus (98%), Zebrafish (92%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CAB39 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human CAB39. The exact sequence is proprietary.

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CAB39 Antibody

Western Blot: CAB39 Antibody [NBP2-15658]

Western Blot: CAB39 Antibody [NBP2-15658]

Western Blot: CAB39 Antibody [NBP2-15658] - Sample (30 ug of whole cell lysate) A: HeLa B: HCT116 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: CAB39 Antibody [NBP2-15658]

Immunocytochemistry/ Immunofluorescence: CAB39 Antibody [NBP2-15658]

Immunocytochemistry/Immunofluorescence: CAB39 Antibody [NBP2-15658] - CAB39 antibody detects CAB39 protein at Cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CAB39 protein stained by CAB39 antibody diluted at 1:500. Blue: Hoechst 33343 staining.
Immunohistochemistry-Paraffin: CAB39 Antibody [NBP2-15658]

Immunohistochemistry-Paraffin: CAB39 Antibody [NBP2-15658]

Immunohistochemistry-Paraffin: CAB39 Antibody [NBP2-15658] - Paraffin-embedded mouse testis. CAB39 antibody diluted at 1:500.
View 1 more images

Applications for CAB39 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CAB39

Mouse protein 25 alpha (MO25 alpha) is a 40-kDa protein that, together with the STE20-related adaptor-alpha (STRAD alpha) pseudo kinase, forms a regulatory complex capable of stimulating the activity of the LKB1 tumor suppressor protein kinase. The latter is mutated in the inherited Peutz-Jeghers cancer syndrome (PJS). MO25 alpha binds directly to a conserved Trp-Glu-Phe sequence at the STRAD alpha C terminus, markedly enhancing binding of STRAD alpha to LKB1 and increasing LKB1 catalytic activity (1). Skeletal muscle contraction results in the phosphorylation and activation of the AMP-activated protein kinase (AMPK) by an upstream kinase (AMPKK). The LKB1-STE-related adaptor (STRAD)-mouse protein 25 (MO25) complex is the major AMPKK in skeletal muscle; however, LKB1-STRAD-MO25 activity is not increased by muscle contraction. This relationship suggests that phosphorylation of AMPK by LKB1-STRAD-MO25 during skeletal muscle contraction may be regulated by allosteric mechanisms (2).

Alternate Names

calcium binding protein 39, calcium-binding protein 39, FLJ22682, MO25CGI-66, Protein Mo25

Gene Symbol

CAB39

UniProt

Q9Y376 (Human)

Additional CAB39 Products

Product Documents for CAB39 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CAB39 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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