Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Guinea Pig, Rabbit

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Flow Cytometry, IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1A

Format

BSA Free

Concentration

1 mg/ml

View all CACNA1S Antibodies »

Product Specifications

Immunogen

Purified rabbit muscle T-tubule dihydropyridine receptor.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Plasma membrane

Specificity

Detects 1,4-dihydropyridine (DHP) receptor alpha-1 subunit in skeletal muscle. The DHP Receptor alpha-1 protein is also known as CACNA1S or Cav1.1 alpha-1 subunit.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CACNA1S Antibody (1A) - BSA Free

Immunohistochemistry-Paraffin: CACNA1S Antibody (1A) - BSA Free [NB300-542]

Immunohistochemistry-Paraffin: CACNA1S Antibody (1A) - BSA Free [NB300-542]

Immunohistochemistry-Paraffin: CACNA1S Antibody (1A) [NB300-542] - Immunohistochemistry was performed on normal biopsies of deparaffinized human skeletal muscle tissue.
Flow Cytometry: CACNA1S Antibody (1A) - BSA Free [NB300-542]

Flow Cytometry: CACNA1S Antibody (1A) - BSA Free [NB300-542]

Flow Cytometry: CACNA1S Antibody (1A) [NB300-542] - Flow cytometry analysis of Dihydropyridine Receptor alpha-1 in U251 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS.

Applications for CACNA1S Antibody (1A) - BSA Free

Application
Recommended Usage

Flow Cytometry

1 ug

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:200

Immunohistochemistry-Paraffin

1:20

Immunoprecipitation

1:10 - 1:500

Western Blot

1:500
Application Notes
WB: Detects an approx. 200 kDa protein representing the DHP receptor in rat skeletal muscle extracts. IHC: Staining of DHP receptor in rabbit skeletal muscle with this antibody results in double rows of discrete punctate staining representing pairs of triads on the opposing sides of the Z-lines.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CACNA1S

Voltage-sensitive calcium channels mediate the entry of calcium into many types of excitable cells and thus play a key role in neurotransmitter release and excitation-contraction (E-C) coupling. The 1,4-dihydropyridines (DHPs) are synthetic organic compounds which can be used to identify the L-type calcium channels that are found in all types of vertebrate muscle, neuronal and neuroendocrine cells. The DHP receptor is part of the L-type calcium channel complex and is thought to be the voltage sensor in E-C coupling.The purified DHP receptor isolated from triads is composed of at least four subunits. The alpha-1 subunit contains the binding site for the DHPs and shows high sequence homology to the voltage gated sodium channel. The alpha-2 subunit is a large glycoprotein associated with the DHP receptor which was first described in skeletal muscle and is also found in high concentrations in other excitable tissues such as cardiac muscle and brain and in low concentrations in most other tissues studied. The other two subunits that co-purify with the DHP receptor are termed beta and gamma.

Long Name

Voltage-dependent L-type calcium channel subunit alpha-1S

Alternate Names

CACH1, CACN1, CACNL1A3, Cav1.1, MHS5

Entrez Gene IDs

779 (Human); 682930 (Rat)

Gene Symbol

CACNA1S

Additional CACNA1S Products

Product Documents for CACNA1S Antibody (1A) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CACNA1S Antibody (1A) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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