Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Microarray, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # N8b/1

Concentration

1 mg/ml

View all CACNB2 Antibodies »

Product Specifications

Immunogen

Synthetic peptide amino acids 189-205 of rat CavBeta2

Localization

Cell Membrane , Sarcolemma

Specificity

Detects approx 78 kDa. No cross reactivity against CavB1, CavB3, CavB4.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CACNB2 Antibody (N8b/1)

Western Blot: CACNB2 Antibody (N8b/1) [NBP1-47607]

Western Blot: CACNB2 Antibody (N8b/1) [NBP1-47607]

Western Blot: CACNB2 Antibody (N8b/1) [NBP1-47607] - Western Blot analysis of Human Cell line lysates showing detection of Cav Beta2 Calcium Channel protein using Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody, Clone S8b-1 (NBP1-47607). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody (NBP1-47607) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunocytochemistry/ Immunofluorescence: CACNB2 Antibody (N8b/1) [NBP1-47607]

Immunocytochemistry/ Immunofluorescence: CACNB2 Antibody (N8b/1) [NBP1-47607]

Immunocytochemistry/Immunofluorescence: CACNB2 Antibody (N8b/1) [NBP1-47607] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav beta 2 Monoclonal Antibody, Clone S8B-1 (NBP1-47607). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Cav beta 2 Monoclonal Antibody (NBP1-47607) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav beta 2 Antibody (D) Composite.
Immunohistochemistry: CACNB2 Antibody (N8b/1) [NBP1-47607]

Immunohistochemistry: CACNB2 Antibody (N8b/1) [NBP1-47607]

Immunohistochemistry: CACNB2 Antibody (N8b/1) [NBP1-47607] - Immunohistochemistry analysis using Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody, Clone S8b-1 (NBP1-47607). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody (NBP1-47607) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: All nuclei. Some nuclei also staining higher up in epidermis.
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Applications for CACNB2 Antibody (N8b/1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:1000

Western Blot

1:1000
Application Notes
1 ug/ml of Cav beta 2 Antibody was sufficient for detection of CavB2 in 10 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CACNB2

Function: The beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting.; Defects in CACNB2 are the cause of Brugada syndrome type 4 (BRS4). BRS4 is a heart disease characterized by the association of Brugada syndrome with shortened QT intervals. Brugada syndrome is a tachyarrhythmia characterized by right bundle branch block and ST segment elevation on an electrocardiogram (ECG). It can cause the ventricles to beat so fast that the blood is prevented from circulating efficiently in the body. When this situation occurs (called ventricular fibrillation), the individual will faint and may die in a few minutes if the heart is not reset.; Subcellular location: Cell membrane sarcolemma; Peripheral membrane protein; Cytoplasmic side; Tissue specificity: Expressed in all tissues.

Long Name

Voltage-dependent L-type calcium channel subunit beta-2

Alternate Names

CAB2, CACNLB2, MYSB

Entrez Gene IDs

116600 (Rat)

Gene Symbol

CACNB2

UniProt

Q8VGC3 (Rat)

Additional CACNB2 Products

Product Documents for CACNB2 Antibody (N8b/1)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CACNB2 Antibody (N8b/1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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