Cadherin-11 Antibody (HL1044)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13699

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-13699
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Feline

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1044

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Cadherin-11 Antibodies »

Product Summary for Cadherin-11 Antibody (HL1044)

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cadherin-11. The exact sequence is proprietary.

Reactivity Notes

Cat (100%).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

88 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Cadherin-11 Antibody (HL1044)

Western Blot: Cadherin-11 Antibody (HL1044) [NBP3-13699]

Western Blot: Cadherin-11 Antibody (HL1044) [NBP3-13699]

Western Blot: Cadherin-11 Antibody (HL1044) [NBP3-13699] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Cadherin-11 antibody [HL1044] (NBP3-13699) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Cadherin-11 Antibody (HL1044)

Cadherin-11 Antibody (HL1044) [NBP3-13699]-Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CDH11 antibody [HL1044] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Cadherin-11 Antibody (HL1044) [NBP3-13699]-Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CDH11 antibody [HL1044] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Cadherin-11 Antibody (HL1044)

Western Blot: Cadherin-11 Antibody (HL1044) [NBP3-13699] -

Western Blot: Cadherin-11 Antibody (HL1044) [NBP3-13699] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Cadherin-11 antibody [HL1044] (NBP3-13699) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
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Applications for Cadherin-11 Antibody (HL1044)

Application
Recommended Usage

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cadherin-11

Cadherin-11 is a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Expression of this particular cadherin in osteoblastic cell lines, and its upregulation during differentiation, suggests a specific function in bone development and maintenance. Cadherin-11 is expressed in osteoblasts and the brain as well as other tissues.Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues.

Alternate Names

Cadherin11, CDH11, CDHOB, OB-Cadherin

Gene Symbol

CDH11

Additional Cadherin-11 Products

Product Documents for Cadherin-11 Antibody (HL1044)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cadherin-11 Antibody (HL1044)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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