Cadherin-16 Antibody (CDH16/1071) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47745

Novus Biologicals, part of Bio-Techne
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NBP2-47745-0.1mg
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Rabbit

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # CDH16/1071

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all Cadherin-16 Antibodies »

Product Specifications

Immunogen

Recombinant full-length human Cadherin-16 protein (Uniprot: O75309)

Localization

Cell Surface with some cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-45157).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Cadherin-16 Antibody (CDH16/1071) - Azide and BSA Free

Western Blot: Cadherin-16 Antibody (CDH16/1071)Azide and BSA Free [NBP2-47745]

Western Blot: Cadherin-16 Antibody (CDH16/1071)Azide and BSA Free [NBP2-47745]

Western Blot: Cadherin-16 Antibody (CDH16/1071) - Azide and BSA Free [NBP2-47745] - Analysis of human kidney tissue lysate usingCadherin-16 Antibody (CDH16/1071).
Immunohistochemistry-Paraffin: Cadherin-16 Antibody (CDH16/1071) - Azide and BSA Free [NBP2-47745]

Immunohistochemistry-Paraffin: Cadherin-16 Antibody (CDH16/1071) - Azide and BSA Free [NBP2-47745]

Immunohistochemistry-Paraffin: Cadherin-16 Antibody (CDH16/1071) - Azide and BSA Free [NBP2-47745] - Human Kidney stained with Cadherin-16 Antibody (CDH16/1071).
Flow Cytometry: Cadherin-16 Antibody (CDH16/1071) - Azide and BSA Free [NBP2-47745]

Flow Cytometry: Cadherin-16 Antibody (CDH16/1071) - Azide and BSA Free [NBP2-47745]

Flow Cytometry: Cadherin-16 Antibody (CDH16/1071) - Azide and BSA Free [NBP2-47745] - Analysis of human HEK293 cells usingCadherin-16 Antibody (CDH16/1071) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
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Applications for Cadherin-16 Antibody (CDH16/1071) - Azide and BSA Free

Application
Recommended Usage

ELISA

2-4 ug/ml

Flow Cytometry

0.5 - 1 ug/million cells in 0.1 ml

Immunohistochemistry-Paraffin

0.5 - 1.0 ug/ml
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Cadherin-16

Cadherin 16 is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. Mapped to a previously identified cluster of cadherin genes on chromosome 16q22.1, the gene localizes with superfamily members CDH1, CDH3, CDH5, CDH8 and CDH11. The protein consists of an extracellular domain containing 6 cadherin domains, a transmembrane region and a truncated cytoplasmic domain but lacks the prosequence and tripeptide HAV adhesion recognition sequence typical of most classical cadherins. Expression is exclusively in kidney, where the protein functions as the principal mediator of homotypic cellular recognition, playing a role in the morphogenic direction of tissue development.

Alternate Names

cadherin 16, KSP-cadherin, cadherin-16, Kidney-specific cadherin, Ksp-cadherin

Gene Symbol

CDH16

Additional Cadherin-16 Products

Product Documents for Cadherin-16 Antibody (CDH16/1071) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cadherin-16 Antibody (CDH16/1071) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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