Calbindin 1/2 Antibody (HL1787) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25390
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1787
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fragment of human Calbindin 1/2
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Calbindin 1/2 Antibody (HL1787) - Azide and BSA Free
Western Blot: Calbindin 1/2 Antibody (HL1787) - Azide and BSA Free [NBP3-25390] -
Western Blot: Calbindin 1/2 Antibody (HL1787) - Azide and BSA Free [NBP3-25390] - Human tissue extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin + Calretinin antibody [HL1787] (NBP3-25390) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Western Blot: Calbindin 1/2 Antibody (HL1787) - Azide and BSA Free [NBP3-25390] -
Western Blot: Calbindin 1/2 Antibody (HL1787) - Azide and BSA Free [NBP3-25390] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin + Calretinin antibody [HL1787] (NBP3-25390) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: Calbindin 1/2 Antibody (HL1787) - Azide and BSA Free [NBP3-25390] -
Western Blot: Calbindin 1/2 Antibody (HL1787) - Azide and BSA Free [NBP3-25390] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin + Calretinin antibody [HL1787] (NBP3-25390) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.
Applications for Calbindin 1/2 Antibody (HL1787) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Assay dependent
Immunohistochemistry-Frozen
Assay dependent
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Calbindin 1/2
Alternate Names
CAB27, CAB29, CAL2, CALB, CALB1, CALB2, calbindin 1, 28kDa, calbindin 2, Calbindin D28, calbindin D29K, calretinin, D-28K, RTVL-H protein
Gene Symbol
CALB1
Additional Calbindin 1/2 Products
Product Documents for Calbindin 1/2 Antibody (HL1787) - Azide and BSA Free
Product Specific Notices for Calbindin 1/2 Antibody (HL1787) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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