Calbindin D-28K Antibody (5A9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-50050

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 5A9

Concentration

1 mg/ml

View all Calbindin D-28K Antibodies »

Product Specifications

Immunogen

Full-length recombinant human Calbindin D-28K. [UniProt# P05937]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Calbindin D-28K Antibody (5A9)

Western Blot: Calbindin D-28K Antibody (5A9) [NBP2-50050]

Western Blot: Calbindin D-28K Antibody (5A9) [NBP2-50050]

Western Blot: Calbindin D-28K Antibody (5A9) [NBP2-50050] - Analysis of different neuronal tissue lysates using mouse mAb to Calbindin D-28K, dilution 1:5,000: [1] protein standard, [2] rat cerebellum, [3] pig hippocampus, and [4] cow cerebellum. Bands at about 26kDa corresponds to the calbindin protein, heavily expressed in the cerebellum but a very minor component of hippocampus.
Immunocytochemistry/ Immunofluorescence: Calbindin D-28K Antibody (5A9) [NBP2-50050]

Immunocytochemistry/ Immunofluorescence: Calbindin D-28K Antibody (5A9) [NBP2-50050]

Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody (5A9) [NBP2-50050] - Adult mouse cortex (Left) and cerebellum (Right) sections were stained with NBP2-50050 in red, and co-stained with rabbit polyclonal anti-RBFOX3/NeuN antibody (NBP1-92716, green). Calbindin is expressed in a subset of interneurons in the cortex (Left) and prominently expressed in the dendrites of Purkinje cells in the cerebellum molecular layer (Right). RBFOX3/NeuN expresses in most neurons; as a result, cells positive for Calbindin appear to be yellow. Insets are high magnification images of the boxed area. Blue is Dapi nucleus staining that labels DNA.
Immunohistochemistry Free-Floating: Calbindin D-28K Antibody (5A9) [NBP2-50050]

Immunohistochemistry Free-Floating: Calbindin D-28K Antibody (5A9) [NBP2-50050]

Immunohistochemistry Free-Floating: Calbindin D-28K Antibody (5A9) [NBP2-50050] - Analysis of rat brain cerebellum section stained with mouse mAb to Calbindin D-28K, dilution 1:2,000 (Red), and costained with rabbit GFAP pAb, dilution 1:5,000 (Green). DAPI staining of nuclear DNA (Blue). Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24hrs, cut to 45uM, and free-floating sections were stained with the above antibodies. The Calbindin D-28K antibody prominently labels the dendrites and perikarya of Purkinje cells in the molecular layer of the cerebellum. The GFAP antibody stains the processes of Bergmann glia in the molecular layer and astroglia in the granular and white matter layers of cerebellum.

Applications for Calbindin D-28K Antibody (5A9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:5000

Immunohistochemistry

1:5000

Immunohistochemistry Free-Floating

1:5000

Western Blot

1:5000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50% PBS, 50% glycerol

Preservative

0.035% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Calbindin D-28K

Calbindin D28k is a member of a large family of intracellular calcium-binding proteins, containing EF-hand calcium binding motifs, and related to calmodulin and troponin-C. The biological roles of Calbindin D28k include calcium regulation and calcium-dependent signalling in neurons and during development. Decreases in Calbindin D28k abundance, or loss of Calbindin D28k immunoreactivity, is found in models of neurological disorders such as epilepsy, and in neurodegenerative diseases. Calbindin antibody is often used as a marker for cerebellum Purkinje cells.

Alternate Names

CAB27, CALB, calbindin, calbindin 1, (28kD), calbindin 1, 28kDa, Calbindin D28, D-28K, RTVL-H protein, Vitamin D-dependent calcium-binding protein, avian-type

Gene Symbol

CALB1

UniProt

P05937

Additional Calbindin D-28K Products

Product Documents for Calbindin D-28K Antibody (5A9)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Calbindin D-28K Antibody (5A9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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