Calbindin D-28K Antibody (CALB1/2782)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07208

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # CALB1/2782

Concentration

0.2 mg/ml

View all Calbindin D-28K Antibodies »

Product Summary for Calbindin D-28K Antibody (CALB1/2782)

Immunogen

Recombinant fragment (around aa 7-96) of human Calbindin D-28K protein (exact sequence is proprietary) (Uniprot: P05937)

Localization

Nuclear and Cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08954)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Calbindin D-28K Antibody (CALB1/2782)

Immunohistochemistry-Paraffin: Calbindin D-28K Antibody (CALB1/2782) [NBP3-07208]

Immunohistochemistry-Paraffin: Calbindin D-28K Antibody (CALB1/2782) [NBP3-07208]

Immunohistochemistry-Paraffin: Calbindin D-28K Antibody (CALB1/2782) [NBP3-07208] - Formalin-fixed, paraffin-embedded human Kidney stained with Calbindin D-28K Mouse Monoclonal Antibody (CALB1/2782).
Protein Array: Calbindin D-28K Antibody (CALB1/2782) [NBP3-07208]

Protein Array: Calbindin D-28K Antibody (CALB1/2782) [NBP3-07208]

Protein Array: Calbindin D-28K Antibody (CALB1/2782) [NBP3-07208] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Calbindin D-28K Mouse Monoclonal Antibody (CALB1/2782).

Applications for Calbindin D-28K Antibody (CALB1/2782)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA).
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Calbindin D-28K

Calbindin D28k is a member of a large family of intracellular calcium-binding proteins, containing EF-hand calcium binding motifs, and related to calmodulin and troponin-C. The biological roles of Calbindin D28k include calcium regulation and calcium-dependent signalling in neurons and during development. Decreases in Calbindin D28k abundance, or loss of Calbindin D28k immunoreactivity, is found in models of neurological disorders such as epilepsy, and in neurodegenerative diseases. Calbindin antibody is often used as a marker for cerebellum Purkinje cells.

Alternate Names

CAB27, CALB, calbindin, calbindin 1, (28kD), calbindin 1, 28kDa, Calbindin D28, D-28K, RTVL-H protein, Vitamin D-dependent calcium-binding protein, avian-type

Gene Symbol

CALB1

UniProt

P05937

Additional Calbindin D-28K Products

Product Documents for Calbindin D-28K Antibody (CALB1/2782)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Calbindin D-28K Antibody (CALB1/2782)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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