Calcineurin A Antibody (2G8)

Novus Biologicals, part of Bio-Techne | Catalog # H00005530-M03

Novus Biologicals, part of Bio-Techne
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H00005530-M03
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Key Product Details

Species Reactivity

Human, Rat, Porcine

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2G8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Calcineurin A Antibodies »

Product Specifications

Immunogen

PPP3CA (NP_000935, 1 a.a. ~ 84 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSEPKAIDPKLSTTDRVVKAVPFPPSHRLTAKEVFDNDGKPRVDILKAHLMKEGRLEESVALRIITEGASILRQEKNLLDIDAP

Specificity

PPP3CA (2G8)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Calcineurin A Antibody (2G8)

Western Blot: Calcineurin A Antibody (2G8) [H00005530-M03]

Western Blot: Calcineurin A Antibody (2G8) [H00005530-M03]

Western Blot: Calcineurin A Antibody (2G8) [H00005530-M03] - Analysis of PPP3CA expression in transfected 293T cell line by PPP3CA monoclonal antibody (M03), clone 2G8. Lane 1: PPP3CA transfected lysate (Predicted MW: 57.7 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Calcineurin A Antibody (2G8) [H00005530-M03]

Immunocytochemistry/ Immunofluorescence: Calcineurin A Antibody (2G8) [H00005530-M03]

Immunocytochemistry/Immunofluorescence: Calcineurin A Antibody (2G8) [H00005530-M03] - Analysis of monoclonal antibody to PPP3CA on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: Calcineurin A Antibody (2G8) [H00005530-M03]

Immunohistochemistry-Paraffin: Calcineurin A Antibody (2G8) [H00005530-M03]

Immunohistochemistry-Paraffin: Calcineurin A Antibody (2G8) [H00005530-M03] - Analysis of monoclonal antibody to PPP3CA on formalin-fixed paraffin-embedded human stomach. Antibody concentration 1.5 ug/ml
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Applications for Calcineurin A Antibody (2G8)

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. It has been used for IHC-P.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Calcineurin A

Calcineurin, a major soluble calmodulin binding protein in the brain, a Ca2+/calmodulin dependent serine/threonine protein phosphatase, with a relatively narrow substrate specificity. This metalloenzyme, also known as phosphatase 2B, is a heterodimer composed of a calmodulin binding, catalytic alpha subunit 61 kDa, calcineurin A) and calcium binding beta subunit (18 kDa, calcineurin B). The Ca2+ binding subunit, calcineurin B, is immunologically conserved from yeast to mammals. The presence of 2 different calcineurin B isoforms (beta 1 and beta 2) has been reported in rat testis. The catalytic subunit of calcineurin, calcineurin A, isolated from different tissues or different organisms, exhibits some immunological heterogeneity. Further, there are at least 2 isoforms of calcineurin A in bovine brain (alpha 1 and alpha 2, 61 and 59 kDa, respectively).

Alternate Names

CALNA, CNA1, PP2B, PP3CA, PPP2B, PPP3CA

Entrez Gene IDs

5530 (Human)

Gene Symbol

PPP3CA

OMIM

114105 (Human)

UniProt

NP_000935 (Human)

Additional Calcineurin A Products

Product Documents for Calcineurin A Antibody (2G8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Calcineurin A Antibody (2G8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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