Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # CALNA/2353

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Calcineurin A Antibodies »

Product Specifications

Immunogen

Recombinant full-length human Calcineurin A protein (Uniprot: Q08209)

Localization

Cytoplasmic and Cell Surface

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07658).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Calcineurin A Antibody (CALNA/2353) - Azide and BSA Free

Immunohistochemistry-Paraffin: Calcineurin A Antibody (CALNA/2353) - Azide and BSA Free [NBP3-08395]

Immunohistochemistry-Paraffin: Calcineurin A Antibody (CALNA/2353) - Azide and BSA Free [NBP3-08395]

Immunohistochemistry-Paraffin: Calcineurin A Antibody (CALNA/2353) - Azide and BSA Free [NBP3-08395] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Calcineurin A Mouse Monoclonal Antibody (CALNA/2353).
Immunohistochemistry-Paraffin: Calcineurin A Antibody (CALNA/2353) - Azide and BSA Free [NBP3-08395]

Immunohistochemistry-Paraffin: Calcineurin A Antibody (CALNA/2353) - Azide and BSA Free [NBP3-08395]

Immunohistochemistry-Paraffin: Calcineurin A Antibody (CALNA/2353) - Azide and BSA Free [NBP3-08395] - Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Calcineurin A Mouse Monoclonal Antibody (CALNA/2353).

Applications for Calcineurin A Antibody (CALNA/2353) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Calcineurin A

Calcineurin, a major soluble calmodulin binding protein in the brain, a Ca2+/calmodulin dependent serine/threonine protein phosphatase, with a relatively narrow substrate specificity. This metalloenzyme, also known as phosphatase 2B, is a heterodimer composed of a calmodulin binding, catalytic alpha subunit 61 kDa, calcineurin A) and calcium binding beta subunit (18 kDa, calcineurin B). The Ca2+ binding subunit, calcineurin B, is immunologically conserved from yeast to mammals. The presence of 2 different calcineurin B isoforms (beta 1 and beta 2) has been reported in rat testis. The catalytic subunit of calcineurin, calcineurin A, isolated from different tissues or different organisms, exhibits some immunological heterogeneity. Further, there are at least 2 isoforms of calcineurin A in bovine brain (alpha 1 and alpha 2, 61 and 59 kDa, respectively).

Alternate Names

CALNA, CNA1, PP2B, PP3CA, PPP2B, PPP3CA

Gene Symbol

PPP3CA

Additional Calcineurin A Products

Product Documents for Calcineurin A Antibody (CALNA/2353) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Calcineurin A Antibody (CALNA/2353) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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