Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Calcineurin A Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 470 and the C-terminus (residue 521) of human Protein Phosphatase 3, Catalytic Subunit, Alpha isoform using the numbering given in entry NP_000935.1 (GeneID 5530).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Calcineurin A Antibody

Western Blot: Calcineurin A Antibody [NB110-40553]

Western Blot: Calcineurin A Antibody [NB110-40553]

Western Blot: Calcineurin A Antibody [NB110-40553] - Detection of human and mouse PPP3CA by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPP3CA antibody NB110-40553 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: Calcineurin A Antibody [NB110-40553]

Immunohistochemistry-Paraffin: Calcineurin A Antibody [NB110-40553]

Immunohistochemistry-Paraffin: Calcineurin A Antibody [NB110-40553] - FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- PPP3CA used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: Calcineurin A Antibody [NB110-40553]

Immunoprecipitation: Calcineurin A Antibody [NB110-40553]

Immunoprecipitation: Calcineurin A Antibody [NB110-40553] - Detection of human Calcineurin A (PPP3CA) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Calcineurin A antibody NB110-40553 used for IP at 3 ug per reaction. Calcineurin A was also immunoprecipitated by rabbit anti-Calcineurin A antibody NB110-40552. For blotting immunoprecipitated Calcineurin A, NB110-40553 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for Calcineurin A Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:5000

Immunohistochemistry-Paraffin

1:1000 - 1:5000

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Calcineurin A

Calcineurin, a major soluble calmodulin binding protein in the brain, a Ca2+/calmodulin dependent serine/threonine protein phosphatase, with a relatively narrow substrate specificity. This metalloenzyme, also known as phosphatase 2B, is a heterodimer composed of a calmodulin binding, catalytic alpha subunit 61 kDa, calcineurin A) and calcium binding beta subunit (18 kDa, calcineurin B). The Ca2+ binding subunit, calcineurin B, is immunologically conserved from yeast to mammals. The presence of 2 different calcineurin B isoforms (beta 1 and beta 2) has been reported in rat testis. The catalytic subunit of calcineurin, calcineurin A, isolated from different tissues or different organisms, exhibits some immunological heterogeneity. Further, there are at least 2 isoforms of calcineurin A in bovine brain (alpha 1 and alpha 2, 61 and 59 kDa, respectively).

Alternate Names

CALNA, CNA1, PP2B, PP3CA, PPP2B, PPP3CA

Entrez Gene IDs

5530 (Human); 19055 (Mouse)

Gene Symbol

PPP3CA

UniProt

NP_000935.1 (Human)

Additional Calcineurin A Products

Product Documents for Calcineurin A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Calcineurin A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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