Caldesmon/CALD1 Antibody (h-CALD)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44421

Novus Biologicals, part of Bio-Techne
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NBP2-44421-0.02mg
NBP2-44421-0.1mg
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # h-CALD

Concentration

0.2 mg/ml

View all Caldesmon/CALD1 Antibodies »

Product Summary for Caldesmon/CALD1 Antibody (h-CALD)

Immunogen

Crude human uterus extract

Localization

Cytoplasmic

Specificity

Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (12080kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This monoclonal antibody recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.

Marker

Smooth Muscle Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

150 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47816)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Caldesmon/CALD1 Antibody (h-CALD)

Western Blot: Caldesmon/CALD1 Antibody (h-CALD) [NBP2-44421]

Western Blot: Caldesmon/CALD1 Antibody (h-CALD) [NBP2-44421]

Western Blot: Caldesmon/CALD1 Antibody (h-CALD) [NBP2-44421] - Western Blot Analysis of human Ovary tissue using Caldesmon/CALD1 antibody (h-CALD).
Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody (h-CALD) [NBP2-44421]

Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody (h-CALD) [NBP2-44421]

Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody (h-CALD) [NBP2-44421] - Analysis of Human uterus tissue using Caldesmon/CALD1 antibody (h-CALD). Image from verified customer review.
Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody (h-CALD) [NBP2-44421]

Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody (h-CALD) [NBP2-44421]

Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody (h-CALD) [NBP2-44421] - Human Uterus stained with Caldesmon Monoclonal Antibody (h-CALD).
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Applications for Caldesmon/CALD1 Antibody (h-CALD)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

1 - 2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Simple Western

10 ug/ml

Western Blot

2-4 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-44421 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Caldesmon/CALD1

Caldesmon is a smooth muscle and nonmuscle regulatory protein that interacts with actin, myosin, tropomyosin, and calmodulin (1,2). Smooth muscle caldesmon is an elongated molecule with a calmodulin, tropomyosin, and actin-binding region at the C-terminus and a myosin-binding domain at the N-terminus (3). Caldesmon stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, caldesmon inhibits the actomyosin ATPase by binding to F-actin. This inhibition is reduced by calcium-calmodulin and is promoted by tropomyosin (4).

Alternate Names

CALD1, CDM, HCAD, LCAD, NAG22

Gene Symbol

CALD1

UniProt

Q05682 (Human)

Additional Caldesmon/CALD1 Products

Product Documents for Caldesmon/CALD1 Antibody (h-CALD)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Caldesmon/CALD1 Antibody (h-CALD)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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