Caldesmon/CALD1 Antibody (SPM168) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47817
![Western Blot: Caldesmon/CALD1 Antibody (SPM168)Azide and BSA Free [NBP2-47817]](https://resources.bio-techne.com/images/products/Caldesmon-CALD1-Antibody-SPM168-Azide-and-BSA-Free-Western-Blot-NBP2-47817-img0002.jpg "Western Blot: Caldesmon/CALD1 Antibody (SPM168)Azide and BSA Free [NBP2-47817]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM168
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Summary for Caldesmon/CALD1 Antibody (SPM168) - Azide and BSA Free
Immunogen
Crude human uterus extract
Localization
Cytoplasmic
Specificity
Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (12080kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This monoclonal antibody recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.
Marker
Smooth Muscle Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
150 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44422).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Caldesmon/CALD1 Antibody (SPM168) - Azide and BSA Free
Western Blot: Caldesmon/CALD1 Antibody (SPM168)Azide and BSA Free [NBP2-47817]
Western Blot: Caldesmon/CALD1 Antibody (SPM168) - Azide and BSA Free [NBP2-47817] - Western Blot Analysis of human Ovary tissue lysate using Caldesmon/CALD1 MAb (SPM168).![Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody (SPM168) - Azide and BSA Free [NBP2-47817]](https://resources.bio-techne.com/images/products/Caldesmon-CALD1-Antibody-SPM168-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47817-img0001.jpg "Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody (SPM168) - Azide and BSA Free [NBP2-47817]")
Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody (SPM168) - Azide and BSA Free [NBP2-47817]
Immunohistochemistry-Paraffin: Caldesmon/CALD1 Antibody (SPM168) - Azide and BSA Free [NBP2-47817] - Human Uterus stained with Caldesmon Monoclonal Antibody (SPM168).Applications for Caldesmon/CALD1 Antibody (SPM168) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells in 0.1 ml
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
1 - 2 ug/ml
Immunohistochemistry-Paraffin
0.25 - 0.5 ug/ml
Western Blot
2-4 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 7.5-8.5, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Caldesmon/CALD1
Alternate Names
CALD1, CDM, HCAD, LCAD, NAG22
Gene Symbol
CALD1
Additional Caldesmon/CALD1 Products
Product Documents for Caldesmon/CALD1 Antibody (SPM168) - Azide and BSA Free
Product Specific Notices for Caldesmon/CALD1 Antibody (SPM168) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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