CALML3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00001

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CALML3 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human CALML3 (Accession#: P27482; Met1-Lys149)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CALML3 Antibody

Western Blot: CALML3 Antibody [NBP3-00001]

Western Blot: CALML3 Antibody [NBP3-00001]

Western Blot: CALML3 Antibody [NBP3-00001] - Anti-CALML3 rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 15 kDa
Immunohistochemistry-Paraffin: CALML3 Antibody [NBP3-00001]

Immunohistochemistry-Paraffin: CALML3 Antibody [NBP3-00001]

Immunohistochemistry-Paraffin: CALML3 Antibody [NBP3-00001] - Immunochemical staining of human CALML3 in human breast carcinoma (from 4 donors) with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Immunoprecipitation: CALML3 Antibody [NBP3-00001]

Immunoprecipitation: CALML3 Antibody [NBP3-00001]

Immunoprecipitation: CALML3 Antibody [NBP3-00001] - CALML3 was immunoprecipitated using: Lane A: 0.5 mg A431 Whole Cell Lysate 2 ul anti-CALML3 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-CALML3 rabbit polyclonal antibody, at 1:1000 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 17 kDa

Applications for CALML3 Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

4-6 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CALML3

CALML3 may be similar to that of authentic calmodulin and may actually compete with calmodulin by binding, withdifferent affinities, to cellular substrates

Alternate Names

calmodulin-like 3, calmodulin-like protein 3, CaM-like protein, CLPCalmodulin-related protein NB-1

Gene Symbol

CALML3

Additional CALML3 Products

Product Documents for CALML3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CALML3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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