CALML5 Antibody (158)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89982
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: CALML5 Antibody (158) [NBP2-89982]](https://resources.bio-techne.com/images/products/CALML5-Antibody-158-Immunocytochemistry-Immunofluorescence-NBP2-89982-img0001.jpg "Immunocytochemistry/ Immunofluorescence: CALML5 Antibody (158) [NBP2-89982]")
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 158
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for CALML5 Antibody (158)
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human CALML5 (Accession#: AAH39172.1; Met 1-Glu 146).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for CALML5 Antibody (158)
Immunocytochemistry/ Immunofluorescence: CALML5 Antibody (158) [NBP2-89982]
Immunocytochemistry/Immunofluorescence: CALML5 Antibody (158) [NBP2-89982] - Staining of Human CALML5 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CALML5 monoclonal antibody (1:60) at 4? overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).![Flow Cytometry: CALML5 Antibody (158) [NBP2-89982]](https://resources.bio-techne.com/images/products/CALML5-Antibody-158-Flow-Cytometry-NBP2-89982-img0002.jpg "Flow Cytometry: CALML5 Antibody (158) [NBP2-89982]")
Flow Cytometry: CALML5 Antibody (158) [NBP2-89982]
Flow Cytometry: CALML5 Antibody (158) [NBP2-89982] - Analysis of Human CALML5 expression on A431 cells. The cells were treated, stained with purified anti-Human CALML5, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.Applications for CALML5 Antibody (158)
Application
Recommended Usage
ELISA
1:5000-1:10000
Flow Cytometry
1:25-1:100
Immunocytochemistry/ Immunofluorescence
1:20-1:100
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CALML5
Alternate Names
calmodulin-like 5, calmodulin-like protein 5, CLSPCalmodulin-like skin protein
Gene Symbol
CALML5
Additional CALML5 Products
Product Documents for CALML5 Antibody (158)
Product Specific Notices for CALML5 Antibody (158)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...