CALML5 Antibody (2F10)

Novus Biologicals, part of Bio-Techne | Catalog # H00051806-M16

Novus Biologicals, part of Bio-Techne
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H00051806-M16
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2F10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CALML5 Antibodies »

Product Specifications

Immunogen

CALML5 (AAH39172, 1 a.a. ~ 146 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAGELTPEEEAQYKKAFSAVDTDGNGTINAQELGAALKATGKNLSEAQLRKLISEVDGDGDGEISFQEFLTAARKARAGLEDLQVAFRAFDQDGDGHITVDELRRAMAGLGQPLPQEELDAMIREADVDQDGRVNYEEFARMLAQE

Specificity

CALML5 - calmodulin-like 5 (2F10)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for CALML5 Antibody (2F10)

Western Blot: CALML5 Antibody (2F10) [H00051806-M16]

Western Blot: CALML5 Antibody (2F10) [H00051806-M16]

Western Blot: CALML5 Antibody (2F10) [H00051806-M16] - Analysis of CALML5 expression in transfected 293T cell line by CALML5 monoclonal antibody (M16), clone 2F10. Lane 1: CALML5 transfected lysatE (15.9 KDa). Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: CALML5 Antibody (2F10) [H00051806-M16]

Immunohistochemistry-Paraffin: CALML5 Antibody (2F10) [H00051806-M16]

Immunohistochemistry-Paraffin: CALML5 Antibody (2F10) [H00051806-M16] - Analysis of monoclonal antibody to CALML5 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml
Sandwich ELISA: CALML5 Antibody (2F10) [H00051806-M16] - Detection limit for recombinant GST tagged CALML5 is 0.03 ng/ml as a capture antibody.

Applications for CALML5 Antibody (2F10)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody Reactive against transfected lysate and Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CALML5

This gene encodes a novel calcium binding protein expressed in the epidermis and related to the calmodulin family of calcium binding proteins. Functional studies with recombinant protein demonstrate it does bind calcium and undergoes a conformational change when it does so. Abundant expression is detected only in reconstructed epidermis and is restricted to differentiating keratinocytes. In addition, it can associate with transglutaminase 3, shown to be a key enzyme in the terminal differentiation of keratinocytes. [provided by RefSeq]

Alternate Names

calmodulin-like 5, calmodulin-like protein 5, CLSPCalmodulin-like skin protein

Entrez Gene IDs

51806 (Human)

Gene Symbol

CALML5

UniProt

AAH39172 (Human)

Additional CALML5 Products

Product Documents for CALML5 Antibody (2F10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CALML5 Antibody (2F10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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