Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bacteria, Bovine, Chicken

Cited:

Human

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2D1

Format

BSA Free

Concentration

1 mg/ml

View all Calmodulin 1 Antibodies »

Product Specifications

Immunogen

Calmodulin purified from Dictyostelium discoideum.

Reactivity Notes

E. coli. Chlamydomonas reinhardtii and Dictyostelium discoideum. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

This detects calmodulin. It does not detect parvalbumin, tropinin, S-100, or myosin light chain kinase (MLCK).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Calmodulin Antibody (2D1) - BSA Free

Western Blot: Calmodulin Antibody (2D1)BSA Free [NB120-2860]

Western Blot: Calmodulin Antibody (2D1)BSA Free [NB120-2860]

Western Blot: Calmodulin Antibody (2D1) [NB120-2860] - Purified Calmodulin using NB120-2860 and NB120-5494.
Immunocytochemistry/ Immunofluorescence: Calmodulin Antibody (2D1) - BSA Free [NB120-2860]

Immunocytochemistry/ Immunofluorescence: Calmodulin Antibody (2D1) - BSA Free [NB120-2860]

Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (2D1) [NB120-2860] - Staining of rat brain.
Immunohistochemistry-Paraffin: Calmodulin Antibody (2D1) - BSA Free [NB120-2860]

Immunohistochemistry-Paraffin: Calmodulin Antibody (2D1) - BSA Free [NB120-2860]

Immunohistochemistry-Paraffin: Calmodulin Antibody (2D1) [NB120-2860] - Normal biopsies of deparaffinized Rat testis tissue.
View 7 more images

Applications for Calmodulin Antibody (2D1) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Flow Cytometry

2 ug / 10^6 cells

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:20

Immunohistochemistry-Paraffin

1:20

Western Blot

1:500
Application Notes
WB: Detects an approx. 17 kDa protein representing calmodulin from Dictyostelium cell lysate. Addition of EDTA to buffers completely inhibits antibody staining. IHC: Staining of calmodulin in Dictyostelium cells with this antibody results in staining of the contractile vacuoles.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Calmodulin 1

Calmodulin is a small, highly conserved calcium binding protein found in all eukaryotic cells. With the capacity to bind up to four calcium ions, this 17 kDa protein acts as an important intracellular receptor for regulatory calcium signals. As it binds calcium, calmodulin undergoes conformational changes which can increase its affinity for target proteins. It acts both directly, through interaction with key target enzymes, and indirectly, via specific kinases. Studies have found that calmodulin participates in the regulation of several biological processes including energy and biosynthetic metabolism, cell motility, exocytosis, cytoskeletal assembly, and intracellular modulation of both cAMP and calcium concentrations.

Alternate Names

CALM1, CALML2, CaM, CAM1, CAMI, CPVT4, DD132

Entrez Gene IDs

24242 (Rat)

Gene Symbol

CALM1

Additional Calmodulin 1 Products

Product Documents for Calmodulin Antibody (2D1) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Calmodulin Antibody (2D1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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