Calnexin Antibody (1563)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43765

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Predicted:

Bovine (94%), Canine (94%), Chicken (91%), Porcine (94%). Backed by our 100% Guarantee.

Applications

Validated:

Dot Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 1563

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Calnexin Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calnexin. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (82%), Xenopus laevis (89%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Calnexin Antibody (1563)

Immunocytochemistry/ Immunofluorescence: Calnexin Antibody (1563) [NBP2-43765]

Immunocytochemistry/ Immunofluorescence: Calnexin Antibody (1563) [NBP2-43765]

Immunocytochemistry/Immunofluorescence: Calnexin Antibody (1563) [NBP2-43765] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Calnexin stained by Calnexin antibody [1563] diluted at 1:500. Red: Calnexin, stained by Calnexin antibody [C3], C-term (GTX109669) diluted at 1:500. Blue: Hoechst 33342 staining.
Western Blot: Calnexin Antibody (1563) [NBP2-43765]

Western Blot: Calnexin Antibody (1563) [NBP2-43765]

Western Blot: Calnexin Antibody (1563) [NBP2-43765] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 7.5 % SDS-PAGE Calnexin antibody [1563] dilution: 1:1000.
Western Blot: Calnexin Antibody (1563) [NBP2-43765]

Western Blot: Calnexin Antibody (1563) [NBP2-43765]

Western Blot: Calnexin Antibody (1563) [NBP2-43765] - Analysis of A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat-2 whole cell lysate/extract 7.5 % SDS-PAGE Calnexin antibody [1563] dilution: 1:500
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Applications for Calnexin Antibody (1563)

Application
Recommended Usage

Dot Blot

Assay dependent

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunoprecipitation

Assay dependent

Western Blot

1:500-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Calnexin

Calnexin, an abundant approx. 90kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its ER counterparts which have a KDEL sequence on their Cterminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into mulit-subunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their monoglycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatability complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7).

Alternate Names

calnexin, CNX, IP90FLJ26570, Major histocompatibility complex class I antigen-binding protein p88, p90

Gene Symbol

CANX

Additional Calnexin Products

Product Documents for Calnexin Antibody (1563)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Calnexin Antibody (1563)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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