Calnexin Antibody (IE2.1C12) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80609

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # IE2.1C12

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Calnexin Antibodies »

Product Specifications

Immunogen

Partial recombinant human Calnexin protein (between amino acids 1-300) [UniProt# P27824]

Localization

Endoplasmic reticulum membrane, Melanosome

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for Calnexin Antibody (IE2.1C12) - Azide and BSA Free

Western Blot: Calnexin Antibody (IE2.1C12)Azide and BSA Free [NBP2-80609]

Western Blot: Calnexin Antibody (IE2.1C12)Azide and BSA Free [NBP2-80609]

Western Blot: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609] - Analysis of 11 kDa Partial Recombinant Human Calnexin protein with Calnexin antibody (clone IE2.1C12) at 0.5 ug/ml concentration. Image from the standard format of this antibody.
Immunocytochemistry: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609]

Immunocytochemistry: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609]

Immunocytochemistry: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609] - Analysis of FFPE tissue section of human placenta using mouse monoclonal Calnexin antibody (clone IE2.1C12) at 7 ug/ml concentration. Image from the standard format of this antibody.
Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609]

Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609]

Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) - Azide and BSA Free [NBP2-80609] - Analysis of FFPE tissue section of normal human kidney using mouse monoclonal Calnexin antibody (clone IE2.1C12) at 7 ug/ml concentration. The cells of Glomeruli developed strong cytoplasmic staining. Image from the standard format of this antibody.
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Applications for Calnexin Antibody (IE2.1C12) - Azide and BSA Free

Application
Recommended Usage

Flow (Intracellular)

5 ug/million cells

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry

7 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Calnexin

Calnexin is a calcium-binding protein which interacts with newly synthesized glycoproteins in ER for facilitating protein assembly and/or the retention within ER of unassembled protein subunits as well as incorrectly folded proteins thereby playing a key role in ER's quality control apparatus. The specificity of calnexin for glycoproteins is defined by a lectin site, which binds an early oligosaccharide intermediate on the folding glycoprotein. When associated with partial T-cell antigen receptor complexes which escape immature thymocytes's ER, calnexin regulates thymocyte maturation process and is also implicated in receptor-mediated endocytosis at synapses. MAPK3/ERK1 phosphorylates calnexin at Ser-565 leading to increased calnexin-ribosomal association and DHHC6 mediated calnexin palmitoylation leads to its preferential localization to perinuclear rough ER. Palmitoylation also facilitate calnexin association with RTC/ribosome-translocon complex that is essential to efficient folding of glycosylated proteins.

Alternate Names

calnexin, CNX, IP90FLJ26570, Major histocompatibility complex class I antigen-binding protein p88, p90

Gene Symbol

CANX

Additional Calnexin Products

Product Documents for Calnexin Antibody (IE2.1C12) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Calnexin Antibody (IE2.1C12) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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