Calpain 1 Antibody (CAPN1/1530)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07387

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # CAPN1/1530

Concentration

0.2 mg/ml

View all Calpain 1 Antibodies »

Product Summary for Calpain 1 Antibody (CAPN1/1530)

Immunogen

Recombinant full-length human Calpain 1 protein (Uniprot: P07384)

Localization

Cytoplasm and Cell Surface

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08456)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Calpain 1 Antibody (CAPN1/1530)

Immunohistochemistry-Paraffin: Calpain 1 Antibody (CAPN1/1530) [NBP3-07387]

Immunohistochemistry-Paraffin: Calpain 1 Antibody (CAPN1/1530) [NBP3-07387]

Immunohistochemistry-Paraffin: Calpain 1 Antibody (CAPN1/1530) [NBP3-07387] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Calpain 1 Mouse Monoclonal Antibody (CAPN1/1530).
Immunohistochemistry-Paraffin: Calpain 1 Antibody (CAPN1/1530) [NBP3-07387]

Immunohistochemistry-Paraffin: Calpain 1 Antibody (CAPN1/1530) [NBP3-07387]

Immunohistochemistry-Paraffin: Calpain 1 Antibody (CAPN1/1530) [NBP3-07387] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Calpain 1 Mouse Monoclonal Antibody (CAPN1/1530).

Applications for Calpain 1 Antibody (CAPN1/1530)

Application
Recommended Usage

ELISA

2-4 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Calpain 1

The calpain (calcium-dependent protease or calcium-activated neutral protease) system consists of two ubiquitous forms of calpain (Mu Calpain and M Calpain), a tissue specific calpain (N Calpain), and a calpain inhibitory protein (calpastatin). The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered calcium homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low-level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death.Mu and M Calpains are heterodimers consisting of 28 kDa and 80 kDa subunits. The 28 kDa subunit is identical in the two isoforms, but the 80 kDa subunits differ with ~50% sequence similarity. 28 kDa / 80 kDa complexes are thought to be inactive proenzymes which, upon binding of calcium, undergo conformational changes that promotes cleavage of the 28 kDa subunit and results in enzyme activation.

Long Name

Calpain-1 catalytic subunit

Alternate Names

Calpain mu-type, CANP 1, CANPL1, CAPN1, EC 3.4.22.52, Micromolar-calpain, muCANP, PIG30

Gene Symbol

CAPN1

UniProt

P07384 (Human)

Additional Calpain 1 Products

Product Documents for Calpain 1 Antibody (CAPN1/1530)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Calpain 1 Antibody (CAPN1/1530)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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