Calpain 1 Antibody (SR2190)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21569

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-21569-100ul
NBP3-21569-50ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR2190

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Calpain 1 Antibodies »

Product Summary for Calpain 1 Antibody (SR2190)

Immunogen

A synthesized peptide derived from human Calpain 1 (Uniprot #: P07384)

Specificity

Detects endogenous levels of total Calpain 1

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Calpain 1 Antibody (SR2190)

Western Blot: Calpain 1 Antibody (SR2190) [NBP3-21569] -

Western Blot: Calpain 1 Antibody (SR2190) [NBP3-21569] - Western blot analysis using NBP3-21569 in Human fetal kidney lysate.

Applications for Calpain 1 Antibody (SR2190)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Calpain 1

The calpain (calcium-dependent protease or calcium-activated neutral protease) system consists of two ubiquitous forms of calpain (Mu Calpain and M Calpain), a tissue specific calpain (N Calpain), and a calpain inhibitory protein (calpastatin). The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered calcium homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low-level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death.Mu and M Calpains are heterodimers consisting of 28 kDa and 80 kDa subunits. The 28 kDa subunit is identical in the two isoforms, but the 80 kDa subunits differ with ~50% sequence similarity. 28 kDa / 80 kDa complexes are thought to be inactive proenzymes which, upon binding of calcium, undergo conformational changes that promotes cleavage of the 28 kDa subunit and results in enzyme activation.

Long Name

Calpain-1 catalytic subunit

Alternate Names

Calpain mu-type, CANP 1, CANPL1, CAPN1, EC 3.4.22.52, Micromolar-calpain, muCANP, PIG30

Gene Symbol

CAPN1

Additional Calpain 1 Products

Product Documents for Calpain 1 Antibody (SR2190)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Calpain 1 Antibody (SR2190)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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