Calpain 1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15670
Key Product Details
Species Reactivity
Human, Zebrafish
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Calpain 1. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Chicken (80%), Rat (87%), Xenopus laevis (86%), Xenopus tropicalis (84%).
Localization
Cytoplasm; Cell membrane
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Calpain 1 Antibody
![Western Blot: Calpain 1 Antibody [NBP2-15670]](https://resources.bio-techne.com/images/products/Calpain-1-Antibody-Western-Blot-NBP2-15670-img0001.jpg "Western Blot: Calpain 1 Antibody [NBP2-15670]")
Western Blot: Calpain 1 Antibody [NBP2-15670]
Western Blot: Calpain 1 Antibody [NBP2-15670] - Sample (30 ug of whole cell lysate) A: Jurkat 7. 5% SDS PAGE gel, diluted at 1:1000.![Immunocytochemistry/ Immunofluorescence: Calpain 1 Antibody [NBP2-15670]](https://resources.bio-techne.com/images/products/Calpain-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15670-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Calpain 1 Antibody [NBP2-15670]")
Immunocytochemistry/ Immunofluorescence: Calpain 1 Antibody [NBP2-15670]
Immunocytochemistry/Immunofluorescence: Calpain 1 Antibody [NBP2-15670] - Confocal immunofluorescence analysis of methanol-fixed A431, using Calpain 1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.![Immunohistochemistry-Paraffin: Calpain 1 Antibody [NBP2-15670]](https://resources.bio-techne.com/images/products/Calpain-1-Antibody-Immunohistochemistry-Paraffin-NBP2-15670-img0002.jpg "Immunohistochemistry-Paraffin: Calpain 1 Antibody [NBP2-15670]")
Immunohistochemistry-Paraffin: Calpain 1 Antibody [NBP2-15670]
Immunohistochemistry-Paraffin: Calpain 1 Antibody [NBP2-15670] - Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using antibody at 1:500 dilution.Applications for Calpain 1 Antibody
Application
Recommended Usage
Flow Cytometry
1:10 - 1:1000
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
10 - 1:500
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Calpain 1
Long Name
Calpain-1 catalytic subunit
Alternate Names
Calpain mu-type, CANP 1, CANPL1, CAPN1, EC 3.4.22.52, Micromolar-calpain, muCANP, PIG30
Gene Symbol
CAPN1
UniProt
Additional Calpain 1 Products
Product Documents for Calpain 1 Antibody
Product Specific Notices for Calpain 1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.