Calpain 1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15671
![Immunohistochemistry-Paraffin: Calpain 1 Antibody [NBP2-15671]](https://resources.bio-techne.com/images/products/Calpain-1-Antibody-Immunohistochemistry-Paraffin-NBP2-15671-img0003.jpg "Immunohistochemistry-Paraffin: Calpain 1 Antibody [NBP2-15671]")
![Immunoprecipitation: Calpain 1 Antibody [NBP2-15671]](https://resources.bio-techne.com/images/products/Calpain-1-Antibody-Immunoprecipitation-NBP2-15671-img0005.jpg "Immunoprecipitation: Calpain 1 Antibody [NBP2-15671]")
Key Product Details
Species Reactivity
Human, Mouse, Squirrel
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Calpain 1. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Rat (88%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Calpain 1 Antibody
![Western Blot: Calpain 1 Antibody [NBP2-15671]](https://resources.bio-techne.com/images/products/Calpain-1-Antibody-Western-Blot-NBP2-15671-img0006.jpg "Western Blot: Calpain 1 Antibody [NBP2-15671]")
Western Blot: Calpain 1 Antibody [NBP2-15671]
Western Blot: Calpain 1 Antibody [NBP2-15671] - Calpain 1 antibody detects Calpain 1 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calpain 1 antibody diluted at a dilution of 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.![Immunocytochemistry/ Immunofluorescence: Calpain 1 Antibody [NBP2-15671]](https://resources.bio-techne.com/images/products/Calpain-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15671-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Calpain 1 Antibody [NBP2-15671]")
Immunocytochemistry/ Immunofluorescence: Calpain 1 Antibody [NBP2-15671]
Immunocytochemistry/Immunofluorescence: Calpain 1 Antibody [NBP2-15671] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.![Immunohistochemistry-Paraffin: Calpain 1 Antibody [NBP2-15671]](https://resources.bio-techne.com/images/products/Calpain-1-Antibody-Immunohistochemistry-Paraffin-NBP2-15671-img0007.jpg "Immunohistochemistry-Paraffin: Calpain 1 Antibody [NBP2-15671]")
Immunohistochemistry-Paraffin: Calpain 1 Antibody [NBP2-15671]
Immunohistochemistry-Paraffin: Calpain 1 Antibody [NBP2-15671] - Mouse lung. Calpain 1 antibody [N3C2], Internal diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.Applications for Calpain 1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Immunoprecipitation
1:100-1:500
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Calpain 1
Long Name
Calpain-1 catalytic subunit
Alternate Names
Calpain mu-type, CANP 1, CANPL1, CAPN1, EC 3.4.22.52, Micromolar-calpain, muCANP, PIG30
Gene Symbol
CAPN1
UniProt
Additional Calpain 1 Products
Product Documents for Calpain 1 Antibody
Product Specific Notices for Calpain 1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.