Calpain 10 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # H00011132-D01P

Novus Biologicals, part of Bio-Techne
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H00011132-D01P
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Calpain 10 Antibodies »

Product Specifications

Immunogen

CAPN10 (NP_075571.1, 1 a.a. - 672 a.a.) full-length human protein. MRAGRGATPARELFRDAAFPAADSSLFCDLSTPLAQFREDITWRRPQEICATPRLFPDDPREGQVKQGLLGDCWFLCACAALQKSRHLLDQVIPPGQPSWADQEYRGSFTCRIWQFGRWVEVTTDDRLPCLAGRLCFSRCQREDVFWLPLLEKVYAKVHGSYEHLWAGQVADALVDLTGGLAERWNLKGVAGSGGQQDRPGRWEHRTCRQLLHLKDQCLISCCVLSPRAGARELGEFHAFIVSDLRELQGQAGQCILLLRIQNPWGRRCWQGLWREGGEGWSQVDAAVASELLSQLQEGEFWVEEEEFLREFDELTVGYPVTEAGHLQSLYTERLLCHTRALPGAWVKGQSAGGCRNNSGFPSNPKFWLRVSEPSEVYIAVLQRSRLHAADWAGRARALVGDSHTSWSPASIPGKHYQAVGLHLWKVEKRRVNLPRVLSMPPVAGTACHAYDREVHLRCELSPGYYLAVPSTFLKDAPGEFLLRVFSTGRVSLSAIRAVAKNTTPGAALPAGEWGTVQLRGSWRVGQTAGGSRNFASYPTNPCFPFSVPEGPGPRCVRITLHQHCRPSDTEFHPIGFHIFQVPEGGRSQDAPPLLLQEPLLSCVPHRYAQEVSRLCLLPAGTYKVVPSTYLPDTEGAFTVTIATRIDRPSIHSQEMLGQFLQEVSVMAVMKT

Specificity

Reacts with calpain 10.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Calpain 10 Antibody

Western Blot: Calpain 10 Antibody [H00011132-D01P]

Western Blot: Calpain 10 Antibody [H00011132-D01P]

Western Blot: Calpain 10 Antibody [H00011132-D01P] - Analysis of CAPN10 expression in transfected 293T cell line by CAPN10 polyclonal antibody.Lane 1: CAPN10 transfected lysate(74.90 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Calpain 10 Antibody [H00011132-D01P]

Immunocytochemistry/ Immunofluorescence: Calpain 10 Antibody [H00011132-D01P]

Immunocytochemistry/Immunofluorescence: Calpain 10 Antibody [H00011132-D01P] - Analysis of antibody to CAPN10 on HeLa cell. Antibody concentration 30 ug/ml.

Applications for Calpain 10 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against transfected lysate in western blot, and as a detection antibody in ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Calpain 10

Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large catalytic subunit has four domains: domain I, the N-terminal regulatory domain that is processed upon calpain activation; domain II, the protease domain; domain III, a linker domain of unknown function; and domain IV, the calmodulin-like calcium-binding domain. This gene encodes a large subunit. It is an atypical calpain in that it lacks the calmodulin-like calcium-binding domain and instead has a divergent C-terminal domain. It is similar in organization to calpains 5 and 6. This gene is associated with type 2 or non-insulin-dependent diabetes mellitus (NIDDM) and located within the NIDDM1 region. Multiple alternative transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Alternate Names

Calcium-activated neutral proteinase 10, calpain 10, calpain-10, calpain-like protease CAPN10, CANP 10, CANP10, EC 3.4.22.-, KIAA1845, NIDDM1

Entrez Gene IDs

11132 (Human)

Gene Symbol

CAPN10

UniProt

NP_075571.1 (Human)

Additional Calpain 10 Products

Product Documents for Calpain 10 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Calpain 10 Antibody

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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