Calpain 10 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-53117

Novus Biologicals, part of Bio-Techne
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NBP1-53117
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all Calpain 10 Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to CAPN10(calpain 10) The peptide sequence was selected from the N terminal of CAPN10. Peptide sequence MRAGRGATPARELFRDAAFPAADSSLFCDLSTPLAQFREDITWRRPQEIC. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for Calpain 10 Antibody

Western Blot: Calpain 10 Antibody [NBP1-53117]

Western Blot: Calpain 10 Antibody [NBP1-53117]

Western Blot: Calpain 10 Antibody [NBP1-53117] - HT1080 cell lysate, concentration 0.2-1 ug/ml.

Applications for Calpain 10 Antibody

Application
Recommended Usage

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Calpain 10

CAPN10 is the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large catalytic subunit has four domains: domain I, the N-terminal regulatory domain that is processed upon calpain activation; domain II, the protease domain; domain III, a linker domain of unknown function; and domain IV, the calmodulin-like calcium-binding domain. This gene encodes a large subunit. It is an atypical calpain in that it lacks the calmodulin-like calcium-binding domain and instead has a divergent C-terminal domain. It is similar in organization to calpains 5 and 6. This gene is associated with type 2 or non-insulin-dependent diabetes mellitus (NIDDM) and located within the NIDDM1 region. Multiple alternative transcript variants encoding different isoforms have been described for this gene.

Alternate Names

Calcium-activated neutral proteinase 10, calpain 10, calpain-10, calpain-like protease CAPN10, CANP 10, CANP10, EC 3.4.22.-, KIAA1845, NIDDM1

Gene Symbol

CAPN10

Additional Calpain 10 Products

Product Documents for Calpain 10 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Calpain 10 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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