Calpain 2 Antibody (OTI1F4) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70335

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey

Applications

CyTOF-ready, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1F4

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Calpain 2 Antibodies »

Product Summary for Calpain 2 Antibody (OTI1F4) - Azide and BSA Free

Immunogen

Full length human recombinant protein of human CAPN2(NP_001739) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

79.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Calpain 2 Antibody (OTI1F4) - Azide and BSA Free

Western Blot: Calpain 2 Antibody (OTI1F4)Azide and BSA Free [NBP2-70335]

Western Blot: Calpain 2 Antibody (OTI1F4)Azide and BSA Free [NBP2-70335]

Western Blot: Calpain 2 Antibody (OTI1F4) - Azide and BSA Free [NBP2-70335] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Calpain 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Calpain 2.
Flow Cytometry: Calpain 2 Antibody (OTI1F4) - Azide and BSA Free [NBP2-70335]

Flow Cytometry: Calpain 2 Antibody (OTI1F4) - Azide and BSA Free [NBP2-70335]

Flow Cytometry: Calpain 2 Antibody (OTI1F4) - Azide and BSA Free [NBP2-70335] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Calpain 2 antibody, and then analyzed by flow cytometry.
Western Blot: Calpain 2 Antibody (OTI1F4)Azide and BSA Free [NBP2-70335]

Western Blot: Calpain 2 Antibody (OTI1F4)Azide and BSA Free [NBP2-70335]

Western Blot: Calpain 2 Antibody (OTI1F4) - Azide and BSA Free [NBP2-70335] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Calpain 2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Applications for Calpain 2 Antibody (OTI1F4) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Western Blot

1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Calpain 2

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammaliancalpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist ofheterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This geneencodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites inthe 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene.(provided by RefSeq)

Alternate Names

Calcium-activated neutral proteinase 2, calpain 2, (m/II) large subunit, calpain 2, large [catalytic] subunit, calpain 2, large subunit, Calpain large polypeptide L2, Calpain M-type, calpain, large polypeptide L2, calpain-2 catalytic subunit, Calpain-2 large subunit, CANP 2, CANPL2CANP2, CANPml, EC 3.4.22, EC 3.4.22.53, FLJ39928, M-calpain, mCANP, millimolar-calpain

Gene Symbol

CAPN2

Additional Calpain 2 Products

Product Documents for Calpain 2 Antibody (OTI1F4) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Calpain 2 Antibody (OTI1F4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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