Calpain S1 Antibody (5X8H4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16711

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16711-100ul
NBP3-16711-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5X8H4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Calpain S1 Antibodies »

Product Summary for Calpain S1 Antibody (5X8H4)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human Calpain S1 (P04632). MFLVNSFLKGGGGGGGGGGGLGGGLGNVLGGLISGAGGGGGGGGGGGGGGGGGGGGTAMRILGGVISAISEAAAQYNPEPPPPRTHYSNIEANESEEVRQ

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Calpain S1 Antibody (5X8H4)

Western Blot: Calpain S1 Antibody (5X8H4) [NBP3-16711]

Western Blot: Calpain S1 Antibody (5X8H4) [NBP3-16711]

Western Blot: Calpain S1 Antibody (5X8H4) [NBP3-16711] - Western blot analysis of extracts of various cell lines, using Calpain S1 Rabbit mAb (NBP3-16711) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Applications for Calpain S1 Antibody (5X8H4)

Application
Recommended Usage

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Calpain S1

This gene is a member of the calpain small subunit family. Calpains are calcium-dependent cysteine proteinases that are widely distributed in mammalian cells. Calpains operate as heterodimers, comprising a specific large catalytic subunit (calpain 1 subunit in Calpain I, and calpain 2 subunit in Calpain II), and a common small regulatory subunit encoded by this gene. This encoded protein is essential for the stability and function of both calpain heterodimers, whose proteolytic activities influence various cellular functions including apoptosis, proliferation, migration, adhesion, and autophagy. Calpains have been implicated in neurodegenerative processes, such as myotonic dystrophy. A pseudogene of this gene has been defined on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

Alternate Names

Calcium-activated neutral proteinase small subunit, Calcium-dependent protease small subunit 1, calcium-dependent protease, small subunit, calpain 4, small subunit (30K), Calpain regulatory subunit, calpain small subunit 1, calpain, small polypeptide, CALPAIN4, CANP, CANPS, CAPN4, CDPS, CSS1, epididymis secretory sperm binding protein

Gene Symbol

CAPNS1

Additional Calpain S1 Products

Product Documents for Calpain S1 Antibody (5X8H4)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Calpain S1 Antibody (5X8H4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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