Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # CAST/1550

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Calpastatin Antibodies »

Product Specifications

Immunogen

Recombinant full-length human Calpastatin protein (Uniprot: P20810)

Localization

Cytoplasm and Cell Surface

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

126 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07397).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Calpastatin Antibody (CAST/1550) - Azide and BSA Free

Immunohistochemistry-Paraffin: Calpastatin Antibody (CAST/1550) - Azide and BSA Free [NBP3-08510]

Immunohistochemistry-Paraffin: Calpastatin Antibody (CAST/1550) - Azide and BSA Free [NBP3-08510]

Immunohistochemistry-Paraffin: Calpastatin Antibody (CAST/1550) - Azide and BSA Free [NBP3-08510] - Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with Calpastatin Mouse Monoclonal Antibody (CAST/1550).
Immunohistochemistry-Paraffin: Calpastatin Antibody (CAST/1550) - Azide and BSA Free [NBP3-08510]

Immunohistochemistry-Paraffin: Calpastatin Antibody (CAST/1550) - Azide and BSA Free [NBP3-08510]

Immunohistochemistry-Paraffin: Calpastatin Antibody (CAST/1550) - Azide and BSA Free [NBP3-08510] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Calpastatin Mouse Monoclonal Antibody (CAST/1550).
Immunohistochemistry-Paraffin: Calpastatin Antibody (CAST/1550) - Azide and BSA Free [NBP3-08510]

Immunohistochemistry-Paraffin: Calpastatin Antibody (CAST/1550) - Azide and BSA Free [NBP3-08510]

Immunohistochemistry-Paraffin: Calpastatin Antibody (CAST/1550) - Azide and BSA Free [NBP3-08510] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Calpastatin Mouse Monoclonal Antibody (CAST/1550).
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Applications for Calpastatin Antibody (CAST/1550) - Azide and BSA Free

Application
Recommended Usage

ELISA

2-4 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
ELISA: Use antibody at 2-4ug/ml. For coating, order antibody without BSA

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Calpastatin

The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered calcium homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low-level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death.Calpastatin, a specific endogenous inhibitor of calpain, has a predicted molecular weight of ~77 kDa and an internal repeat of four homologous domains which allow it to inhibit multiple calpain molecules simultaneously. It functions by binding to calpain when calpain binds calcium. When Calpastatin is subjected to proteolytic digestion, fragments of calpastatin as small as 15 kDa still retain inhibitory activity.

Alternate Names

BS-17, Calpain inhibitor, calpastatin, MGC9402, Sperm BS-17 component

Gene Symbol

CAST

Additional Calpastatin Products

Product Documents for Calpastatin Antibody (CAST/1550) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Calpastatin Antibody (CAST/1550) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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