Calpastatin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38336

Novus Biologicals, part of Bio-Techne
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NBP2-38336-25ul
NBP2-38336
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Calpastatin Antibodies »

Product Summary for Calpastatin Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: GPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Calpastatin Antibody

Immunocytochemistry/ Immunofluorescence: Calpastatin Antibody [NBP2-38336]

Immunocytochemistry/ Immunofluorescence: Calpastatin Antibody [NBP2-38336]

Immunocytochemistry/Immunofluorescence: Calpastatin Antibody [NBP2-38336] - Staining of human cell line A549 shows positivity in plasma membrane and cytoplasm.
Immunohistochemistry: Calpastatin Antibody [NBP2-38336]

Immunohistochemistry: Calpastatin Antibody [NBP2-38336]

Immunohistochemistry: Calpastatin Antibody [NBP2-38336] - Staining of human prostate shows strong cytoplasmic and membranous positivity in glandular cells.

Applications for Calpastatin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Calpastatin

The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered calcium homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low-level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death.Calpastatin, a specific endogenous inhibitor of calpain, has a predicted molecular weight of ~77 kDa and an internal repeat of four homologous domains which allow it to inhibit multiple calpain molecules simultaneously. It functions by binding to calpain when calpain binds calcium. When Calpastatin is subjected to proteolytic digestion, fragments of calpastatin as small as 15 kDa still retain inhibitory activity.

Alternate Names

BS-17, Calpain inhibitor, calpastatin, MGC9402, Sperm BS-17 component

Gene Symbol

CAST

UniProt

P20810 (Human)

Additional Calpastatin Products

Product Documents for Calpastatin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Calpastatin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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