Calponin 1 Antibody (1H5B5) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52445

Novus Biologicals, part of Bio-Techne
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NBP2-52445-0.025mg
NBP2-52445
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1H5B5

Format

BSA Free

Concentration

1 mg/ml

View all Calponin 1 Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Calponin 1 (AA: 16-165) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

33.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Calponin 1 Antibody (1H5B5) - BSA Free

Western Blot: Calponin 1 Antibody (1H5B5)BSA Free [NBP2-52445]

Western Blot: Calponin 1 Antibody (1H5B5)BSA Free [NBP2-52445]

Western Blot: Calponin 1 Antibody (1H5B5) [NBP2-52445] - Analysis using CNN1 mAb against human CNN1 (AA: 16-165) recombinant protein. (Expected MW is 43.1 kDa)
Immunohistochemistry-Paraffin: Calponin 1 Antibody (1H5B5) - BSA Free [NBP2-52445]

Immunohistochemistry-Paraffin: Calponin 1 Antibody (1H5B5) - BSA Free [NBP2-52445]

Immunohistochemistry-Paraffin: Calponin 1 Antibody (1H5B5) [NBP2-52445] - Analysis of paraffin-embedded cervical cancer tissues using CNN1 mouse mAb with DAB staining.
Western Blot: Calponin 1 Antibody (1H5B5)BSA Free [NBP2-52445]

Western Blot: Calponin 1 Antibody (1H5B5)BSA Free [NBP2-52445]

Western Blot: Calponin 1 Antibody (1H5B5) [NBP2-52445] - Analysis using CNN1 mAb against HEK293 (1) and CNN1 (AA: 16-165)-hIgGFc transfected HEK293 (2) cell lysate.
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Applications for Calponin 1 Antibody (1H5B5) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Calponin 1

Calponin is a smooth muscle specific, actin-, tropomyosin- and calmodulin-binding protein thought to be involved in regulation of actomyosin as well as the regulation or modulation of contraction (1). Calponin binds filamentous actin (F-actin) through two distinct sites ABS1 and ABS2, with an affinity in the low micromolar range (2) Immunoreactivity for calponin, along with alpha-smooth muscle actin and smooth muscle myosin heavy chains, confirms the known neoplastic myoepithelial component of adenoid cystic carcinomas and epithelial-myoepithelial carcinomas. The consistently positive staining pattern in adenoid cystic carcinomas may be diagnostically useful in discriminating histologically similar but consistently negative polymorphous low-grade adenocarcinomas (3).

Alternate Names

Calponin H1, CNN1, Sm-Calp, SMCC

Gene Symbol

CNN1

Additional Calponin 1 Products

Product Documents for Calponin 1 Antibody (1H5B5) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Calponin 1 Antibody (1H5B5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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