Calponin 1 Antibody (2H1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26649
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2H1
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Calponin 1 Antibody (2H1)
Immunogen
A synthesized peptide derived from Human Calponin 1 [UniProt P51911]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Calponin 1 Antibody (2H1)
Immunohistochemistry: Calponin 1 Antibody (2H1) [NBP3-26649] -
Immunohistochemistry: Calponin 1 Antibody (2H1) [NBP3-26649] - Image of Calponin 1 Antibody (2H1) diluted at 1:105 and staining in paraffin-embedded human heart tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot: Calponin 1 Antibody (2H1) [NBP3-26649] -
Western Blot: Calponin 1 Antibody (2H1) [NBP3-26649] - Positive Western Blot detected in: 293 whole cell lysate, Mouse lung tissue.All lanes: Calponin 1 Antibody at 1.05ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 34, 32 KDa
Observed band size: 34 KDa
Applications for Calponin 1 Antibody (2H1)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: Calponin 1
Alternate Names
Calponin H1, CNN1, Sm-Calp, SMCC
Gene Symbol
CNN1
Additional Calponin 1 Products
Product Documents for Calponin 1 Antibody (2H1)
Product Specific Notices for Calponin 1 Antibody (2H1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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