Calponin 1 Antibody (CNN1/4227R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07589
Recombinant Monoclonal Antibody
![Immunohistochemistry-Paraffin: Calponin 1 Antibody (CNN1/4227R) [NBP3-07589]](https://resources.bio-techne.com/images/products/Calponin-1-Antibody-CNN1-4227R-Immunohistochemistry-Paraffin-NBP3-07589-img0002.jpg "Immunohistochemistry-Paraffin: Calponin 1 Antibody (CNN1/4227R) [NBP3-07589]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # CNN1/4227R
Concentration
0.2 mg/ml
Product Summary for Calponin 1 Antibody (CNN1/4227R)
Immunogen
Synthetic peptide corresponding to residues on the C-terminus of human Calponin 1 was used as an immunogen. (Uniprot: P51911)
Localization
Cytoplasmic
Specificity
Calponin a 33 kDa polypeptide, is a cytoskeleton-associated actin-binding protein that also interacts tropomyosin and calmodulin. Calponin antibody has been found to be useful as a marker for myoepithelial and basal lamina in differentiating microinvasive from in situ ductal carcinomas of the breast. Calponin antibody may also have applications in malignant myoepithelium and pleomorphic adenoma of salivary gland as well as a useful marker for fine needle aspirates of papillary breast lesions. The antibody has been found in tissue extracts from smooth muscle, but not in fibroblast extracts.
Marker
Smooth Muscle Marker
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08462)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for Calponin 1 Antibody (CNN1/4227R)
Immunohistochemistry-Paraffin: Calponin 1 Antibody (CNN1/4227R) [NBP3-07589]
Immunohistochemistry-Paraffin: Calponin 1 Antibody (CNN1/4227R) [NBP3-07589] - Formalin-fixed, paraffin-embedded human leiomyosarcoma stained with Calponin 1 Rabbit Recombinant Monoclonal Antibody (CNN1/4227R).![Immunohistochemistry-Paraffin: Calponin 1 Antibody (CNN1/4227R) [NBP3-07589]](https://resources.bio-techne.com/images/products/Calponin-1-Antibody-CNN1-4227R-Immunohistochemistry-Paraffin-NBP3-07589-img0001.jpg "Immunohistochemistry-Paraffin: Calponin 1 Antibody (CNN1/4227R) [NBP3-07589]")
Immunohistochemistry-Paraffin: Calponin 1 Antibody (CNN1/4227R) [NBP3-07589]
Immunohistochemistry-Paraffin: Calponin 1 Antibody (CNN1/4227R) [NBP3-07589] - Formalin-fixed, paraffin-embedded human leiomyosarcoma stained with Calponin 1 Rabbit Recombinant Monoclonal Antibody (CNN1/4227R).Applications for Calponin 1 Antibody (CNN1/4227R)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Calponin 1
Additional Calponin 1 Products
Product Documents for Calponin 1 Antibody (CNN1/4227R)
Product Specific Notices for Calponin 1 Antibody (CNN1/4227R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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