Calponin 1 Antibody (SPM169)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44480

Novus Biologicals, part of Bio-Techne
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NBP2-44480-0.02mg
NBP2-44480-0.1mg
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Key Product Details

Species Reactivity

Human, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM169

Concentration

0.2 mg/ml

View all Calponin 1 Antibodies »

Product Specifications

Immunogen

Crude human uterus extract

Localization

Cytoplasmic

Specificity

Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist; a 34kDa form and a 29kDa form. Expression of the 29kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this monoclonal antibody reacts with only the 34kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.

Marker

Smooth Muscle Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47713)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Calponin 1 Antibody (SPM169)

Immunocytochemistry/ Immunofluorescence: Calponin 1 Antibody (SPM169) [NBP2-44480]

Immunocytochemistry/ Immunofluorescence: Calponin 1 Antibody (SPM169) [NBP2-44480]

Immunocytochemistry/Immunofluorescence: Calponin 1 Antibody (SPM169) [NBP2-44480] - Analysis of PFA-fixed K562 cells labeling Calponin 1 Antibody (SPM169) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot.
Immunohistochemistry-Paraffin: Calponin 1 Antibody (SPM169) [NBP2-44480]

Immunohistochemistry-Paraffin: Calponin 1 Antibody (SPM169) [NBP2-44480]

Immunohistochemistry-Paraffin: Calponin 1 Antibody (SPM169) [NBP2-44480] - Human Uterus stained with Calponin-1 Monoclonal Antibody (SPM169).
Flow Cytometry: Calponin 1 Antibody (SPM169) [NBP2-44480]

Flow Cytometry: Calponin 1 Antibody (SPM169) [NBP2-44480]

Flow Cytometry: Calponin 1 Antibody (SPM169) [NBP2-44480] - Analysis of PFA-fixed K562 cells using Calponin 1 Antibody (SPM169) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)

Applications for Calponin 1 Antibody (SPM169)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 7.5-8.5, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Calponin 1

Calponin is a smooth muscle specific, actin-, tropomyosin- and calmodulin-binding protein thought to be involved in regulation of actomyosin as well as the regulation or modulation of contraction (1). Calponin binds filamentous actin (F-actin) through two distinct sites ABS1 and ABS2, with an affinity in the low micromolar range (2) Immunoreactivity for calponin, along with alpha-smooth muscle actin and smooth muscle myosin heavy chains, confirms the known neoplastic myoepithelial component of adenoid cystic carcinomas and epithelial-myoepithelial carcinomas. The consistently positive staining pattern in adenoid cystic carcinomas may be diagnostically useful in discriminating histologically similar but consistently negative polymorphous low-grade adenocarcinomas (3).

Alternate Names

Calponin H1, CNN1, Sm-Calp, SMCC

Gene Symbol

CNN1

UniProt

P51911 (Human)

Additional Calponin 1 Products

Product Documents for Calponin 1 Antibody (SPM169)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Calponin 1 Antibody (SPM169)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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