Calretinin Antibody (3G9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-50042
![Western Blot: Calretinin Antibody (3G9) [NBP2-50042]](https://resources.bio-techne.com/images/products/Calretinin-Antibody-3G9-Western-Blot-NBP2-50042-img0002.jpg "Western Blot: Calretinin Antibody (3G9) [NBP2-50042]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 3G9
Concentration
1 mg/ml
Product Summary for Calretinin Antibody (3G9)
Immunogen
Full-length recombinant human Calretinin expressed in and purified from E. coli. [UniProt# P22676]
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Calretinin Antibody (3G9)
Western Blot: Calretinin Antibody (3G9) [NBP2-50042]
Western Blot: Calretinin Antibody (3G9) [NBP2-50042] - Analysis of recombinant protein solutions using mouse mAb to calretinin, NBP2-50042, dilution 1:1,000: [1] protein standard, [2] calbindin, [3] calretinin, [4] parvalbumin, [5] secretogogin. This antibody reacts only with calretinin, and does not bind to other related calcium-binding proteins.![Immunohistochemistry Free-Floating: Calretinin Antibody (3G9) [NBP2-50042]](https://resources.bio-techne.com/images/products/Calretinin-Antibody-3G9-Immunocytochemistry-Immunofluorescence-NBP2-50042-img0001.jpg "Immunohistochemistry Free-Floating: Calretinin Antibody (3G9) [NBP2-50042]")
Immunohistochemistry Free-Floating: Calretinin Antibody (3G9) [NBP2-50042]
Immunohistochemistry Free-Floating: Calretinin Antibody (3G9) [NBP2-50042] - Analysis of rat cerebellum section stained with mouse mAb to calretinin, NBP2-50042, dilution 1:500 in green, and costained with chicken pAb to calbindin, dilution 1:2,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with the above antibodies. The calretinin antibody labels interneurons in the granule cell layer, while the calbindin antibody stains Purkinje neurons and their dendritic processes in the molecular layer of the cerebellum.Applications for Calretinin Antibody (3G9)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:1000
Immunohistochemistry
1:1000
Western Blot
1:1000 - 1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
50% PBS, 50% glycerol
Preservative
0.035% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Calretinin
Additional Calretinin Products
Product Documents for Calretinin Antibody (3G9)
Product Specific Notices for Calretinin Antibody (3G9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...