Calretinin Antibody (6A9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05530

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Bovine, Equine

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgA Clone # 6A9

Concentration

1 mg/ml

View all Calretinin Antibodies »

Product Specifications

Immunogen

Full-length recombinant human Calretinin expressed in and purified from E. coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgA

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Calretinin Antibody (6A9)

Western Blot: Calretinin Antibody [NBP3-05530]

Western Blot: Calretinin Antibody [NBP3-05530]

Western Blot: Calretinin Antibody [NBP3-05530] - Western blot analysis of tissue lysates probed with Calretinin Antibody at a at a dilution of of 1:2,000, in red: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, and [6] cow spinal cord. The single clean band at 29kDa corresponds to the calretinin protein.
Immunocytochemistry/ Immunofluorescence: Calretinin Antibody [NBP3-05530]

Immunocytochemistry/ Immunofluorescence: Calretinin Antibody [NBP3-05530]

Immunocytochemistry/Immunofluorescence: Calretinin Antibody [NBP3-05530] - Immunofluorescent analysis of a section of rat hippocampus section stained with Calretinin Antibody at a at a dilution of of 1:2,000 in green and costained with chicken pAb to parvalbumin at a at a dilution of of 1:1,000, in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The calretinin and parvalbumin antibodies stain different subsets of GABAergic interneurons.

Applications for Calretinin Antibody (6A9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:2000-5000

Immunohistochemistry

1:2000-5000

Western Blot

1:2000-5000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 50% Glycerol

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.

Background: Calretinin

Calretinin (CAB29, calbindin 2) is a calcium binding protein that belongs to calbindin family. It is mainly found in neuronal tissue, high expressed in auditory neurons. It has been implicated in as a calcium buffer, calcium sensor, and as a regulator of apoptosis (1). Calretinin is considered as a specific marker of mesothelllal cells; it is the only marker that has the utility in distinguishing between sarcomatoid mesotheliomas and sarcomatoid renal cell carcinomas (2). It is widely used in the panel of makers for distinguishing mesothlioma from adenocarcinoma. Along with calretinin, the panel includes E-cadherin, CEA, and cytokeratin 5/6 (3).

Alternate Names

CAB29, CAL2, CALB2

Gene Symbol

CALB2

Additional Calretinin Products

Product Documents for Calretinin Antibody (6A9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Calretinin Antibody (6A9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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