Calretinin Antibody (6A9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05530
![Western Blot: Calretinin Antibody [NBP3-05530]](https://resources.bio-techne.com/images/products/Calretinin-Antibody-Western-Blot-NBP3-05530-img0002.jpg "Western Blot: Calretinin Antibody [NBP3-05530]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Bovine, Equine
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgA Clone # 6A9
Concentration
1 mg/ml
Product Summary for Calretinin Antibody (6A9)
Immunogen
Full-length recombinant human Calretinin expressed in and purified from E. coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgA
Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Calretinin Antibody (6A9)
Western Blot: Calretinin Antibody [NBP3-05530]
Western Blot: Calretinin Antibody [NBP3-05530] - Western blot analysis of tissue lysates probed with Calretinin Antibody at a at a dilution of of 1:2,000, in red: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, and [6] cow spinal cord. The single clean band at 29kDa corresponds to the calretinin protein.![Immunocytochemistry/ Immunofluorescence: Calretinin Antibody [NBP3-05530]](https://resources.bio-techne.com/images/products/Calretinin-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05530-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Calretinin Antibody [NBP3-05530]")
Immunocytochemistry/ Immunofluorescence: Calretinin Antibody [NBP3-05530]
Immunocytochemistry/Immunofluorescence: Calretinin Antibody [NBP3-05530] - Immunofluorescent analysis of a section of rat hippocampus section stained with Calretinin Antibody at a at a dilution of of 1:2,000 in green and costained with chicken pAb to parvalbumin at a at a dilution of of 1:1,000, in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The calretinin and parvalbumin antibodies stain different subsets of GABAergic interneurons.Applications for Calretinin Antibody (6A9)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:2000-5000
Immunohistochemistry
1:2000-5000
Western Blot
1:2000-5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS, 50% Glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
Background: Calretinin
Alternate Names
CAB29, CAL2, CALB2
Gene Symbol
CALB2
Additional Calretinin Products
Product Documents for Calretinin Antibody (6A9)
Product Specific Notices for Calretinin Antibody (6A9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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