Calretinin Antibody (CALB2/7029R) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14032
Recombinant Monoclonal Antibody
![Protein Array: Calretinin Antibody (CALB2/7029R) - Azide and BSA Free [NBP3-14032]](https://resources.bio-techne.com/images/products/Calretinin-Antibody-CALB2-7029R-Azide-and-BSA-Free-Protein-Array-NBP3-14032-img0001.jpg "Protein Array: Calretinin Antibody (CALB2/7029R) - Azide and BSA Free [NBP3-14032]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry-Paraffin, Protein Array, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # CALB2/7029R
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for Calretinin Antibody (CALB2/7029R) - Azide and BSA Free
Immunogen
Recombinant human Calretinin protein fragment (around aa23-242) (exact sequence is proprietary) (Uniprot: P22676 )
Localization
Cytoplasm, Nucleus
Specificity
It recognizes a protein of about 29kDa, which is identified as Calretinin (also known as Calbindin 2). Calretinin is a vitamin D-dependent calcium-binding protein involved in calcium signaling. It is present in subsets of neurons throughout the brain and spinal chord, including sensory ganglia. Antibody to calretinin is useful in differentiating mesothelioma from adenocarcinomas of the lung. It also aids in differentiating adrenal cortical neoplasms from pheochromocytomas.
Marker
Mesothelioma Marker
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS WITHOUT BSA. Also available at 200 ug/ml WITH BSA & azide (NBP3-13799).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for Calretinin Antibody (CALB2/7029R) - Azide and BSA Free
Protein Array: Calretinin Antibody (CALB2/7029R) - Azide and BSA Free [NBP3-14032]
Protein Array: Calretinin Antibody (CALB2/7029R) - Azide and BSA Free [NBP3-14032] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Calretinin Antibody (CALB2/7029R).
Calretinin Antibody (CALB2/7029R)
Formalin-fixed, paraffin-embedded human brain stained with Calretinin antibody (CALB2/7029R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications for Calretinin Antibody (CALB2/7029R) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Calretinin
Alternate Names
CAB29, CAL2, CALB2
Gene Symbol
CALB2
Additional Calretinin Products
Product Documents for Calretinin Antibody (CALB2/7029R) - Azide and BSA Free
Product Specific Notices for Calretinin Antibody (CALB2/7029R) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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