Calretinin Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30050

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Rat, Guinea Pig

Predicted:

Human (%), Mouse (%), Primate (%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Calretinin Antibodies »

Product Summary for Calretinin Antibody - Azide and BSA Free

Immunogen

Purified guinea pig calretinin. Accession # P47728

Reactivity Notes

Tested in Rat. It is expected that this antibody will also react with human, mouse and non-human primates.

Specificity

Specific for the ~29 kDa Calretinin protein in Western blots. The antibody also works well for immunohistochemistry on formalin or paraformaldehyde perfused rat brain tissue sections.

Marker

Mesothelial Cell Marker

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).

Scientific Data Images for Calretinin Antibody - Azide and BSA Free

Western Blot: Calretinin AntibodyAzide and BSA Free [NBP1-30050]

Western Blot: Calretinin AntibodyAzide and BSA Free [NBP1-30050]

Western Blot: Calretinin Antibody [NBP1-30050] - Rat cerebellum (CB) lysate showing specific immunolabeling of the ~ 29k calretinin protein.
Immunohistochemistry: Calretinin Antibody - Azide and BSA Free [NBP1-30050]

Immunohistochemistry: Calretinin Antibody - Azide and BSA Free [NBP1-30050]

Immunohistochemistry: Calretinin Antibody [NBP1-30050] - Immunostaining of rat cerebellum showing fluorescence of calretinin containing cells.

Applications for Calretinin Antibody - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10 - 1:500

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

10mM Tris and 0.1M NaCl

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Calretinin

Calbindin 2 (calretinin), closely related to calbindin 1, is an intracellular calcium-binding protein belonging to the troponin C superfamily. Calbindin 1 is known to be involved in the vitamin-D-dependent calcium absorption through intestinal and renal epithelia, while the function of neuronal calbindin 1 and calbindin 2 is poorly understood. The sequence of the calbindin 2 cDNA reveals an open reading frame of 271 codons coding for a protein of 31,520 Da, and shares 58% identical residues with human calbindin 1. Calbindin 2 contains five presumably active and one presumably inactive calcium-binding domains. Comparison with the partial sequences available for chick and guinea pig calbindin 2 reveals that the protein is highly conserved in evolution. The calbindin 2 message was detected in the brain, while absent from heart muscle, kidney, liver, lung, spleen, stomach and thyroid gland. There are two additional forms of alternatively spliced calbindin 2 mRNAs encoding C-terminally truncated proteins. Exon 7 can splice to exon 9, resulting in a frame shift and a translational stop at the second codon of exon 9, and encoding calretinin-20k. Exon 7 can also splice to exon 10, resulting in a frame shift and a translational stop at codon 15 of exon 10, and encoding calretinin-22k. The truncated proteins are able to bind calcium.

Alternate Names

CAB29, CAL2, CALB2

Gene Symbol

CALB2

UniProt

P47728 (Rat)

Additional Calretinin Products

Product Documents for Calretinin Antibody - Azide and BSA Free

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Product Specific Notices for Calretinin Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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