Calretinin Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-50029
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Bovine, Equine
Cited:
Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Western Blot
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Chicken IgY
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant full length human Calretinin. [UniProt# P22676]
Specificity
This antibody does not cross-react with the related calcium binding proteins Calbindin D-28K and Parvalbumin.
Clonality
Polyclonal
Host
Chicken
Isotype
IgY
Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Calretinin Antibody
Western Blot: Calretinin Antibody [NBP2-50029]
Western Blot: Calretinin Antibody [NBP2-50029] - Western blot analysis of different tissue lysates using chicken pAb to calretinin, NBP2-50029, dilution 1:1,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, and [6] cow spinal cord. A band at 29kDa corresponds to calretinin protein. The same blot was simultaneously probed with mouse mAb to alpha-nternexin, dilution 1:10,000 in red that reveals the alpha-internexin protein with apparent molecular weight of 66kDa.Immunohistochemistry Free-Floating: Calretinin Antibody [NBP2-50029]
Immunohistochemistry Free-Floating: Calretinin Antibody [NBP2-50029] - Analysis of rat hippocampus section stained with chicken pAb to calretinin, NBP2-50029, dilution 1:1,000 in green, and costained with mouse mAb to parvalbumin, 1:1,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with the above antibodies. The calretinin and parvalbumin antibodies label different classes of interneurons.Immunohistochemistry: Calretinin Antibody [NBP2-50029] -
Immunohistochemistry: Calretinin Antibody [NBP2-50029] - Ketamine treatment increases the activity of immature DG neurons. Representative IHC staining of EGR1+ (red) and Calretinin (CR)+ (green) cells in the DG. White arrows depict example double-positive cells. Scale bars 50 m. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35551462/) licensed under a CC-BY license.Applications for Calretinin Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:1000 - 1:5000
Immunohistochemistry
1:1000-1:5000
Western Blot
1:1000 - 1:5000
Application Notes
In Western blot, this antibody recognizes a clear band at ~29 kDa in rat brain lysates and it reacts only with Calretinin protein, and not other calcium-binding proteins.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgY purified
Formulation
Supplied as concentrated total IgY preparation from egg yolk. Exact concentration of target specific IgY is not quantifiable as the preparation contains both immune IgY specific for the target and also irrelevant, non-immune IgY.
Preservative
0.035% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Calretinin
Additional Calretinin Products
Product Documents for Calretinin Antibody
Product Specific Notices for Calretinin Antibody
Chicken products cannot be exported to Canada.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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